The role of anti-elastin antibodies in a mouse model of asthma.

Autor:	Wakayama T; Department of Respiratory Medicine and Clinical Immunology Dokkyo Medical University, Saitama Medical Center, Saitama, Japan., Sugiyama K; Department of Respiratory Medicine and Clinical Immunology Dokkyo Medical University, Saitama Medical Center, Saitama, Japan.; National Hospital Organization, Utsunomiya National Hospital, Tochigi, Japan., Tokita S; Department of Respiratory Medicine and Clinical Immunology Dokkyo Medical University, Saitama Medical Center, Saitama, Japan., Arifuku H; Department of Respiratory Medicine and Clinical Immunology Dokkyo Medical University, Saitama Medical Center, Saitama, Japan., Otsuji N; Department of Respiratory Medicine and Clinical Immunology Dokkyo Medical University, Saitama Medical Center, Saitama, Japan., Sugitate K; Department of Respiratory Medicine and Clinical Immunology Dokkyo Medical University, Saitama Medical Center, Saitama, Japan., Owada T; Department of Respiratory Medicine and Clinical Immunology Dokkyo Medical University, Saitama Medical Center, Saitama, Japan., Koyama K; Department of Respiratory Medicine and Clinical Immunology Dokkyo Medical University, Saitama Medical Center, Saitama, Japan., Hirata H; Department of Respiratory Medicine and Clinical Immunology Dokkyo Medical University, Saitama Medical Center, Saitama, Japan., Arima M; Department of Rheumatology, Dokkyo Medical University, Tochigi, Japan., Fukushima Y; Department of Respiratory Medicine and Clinical Immunology Dokkyo Medical University, Saitama Medical Center, Saitama, Japan.
Jazyk:	angličtina
Zdroj:	Asian Pacific journal of allergy and immunology [Asian Pac J Allergy Immunol] 2021 Sep 05. Date of Electronic Publication: 2021 Sep 05.
DOI:	10.12932/AP-020221-1053
Abstrakt:	Background: The role of anti-elastin antibody (Ab) in the lung is unclear, although they may be involved in chronic obstructive pulmonary disease (COPD). Recently, increased anti-elastin Ab levels were reported in asthma. Objective: To elucidate the role of anti-elastin Ab in asthma, we created a murine asthma model. Anti-elastin Ab in the airway was neutralized by intratracheal administration of elastin peptide, and the inhibitory effects of anti-elastin Ab on airway remodeling were evaluated. Methods: BALB/c mice were immunized with ovalbumin (OVA) on days 0 and 14. After immunization, the mice received booster OVA via inhalation twice per week for 9 weeks, and bronchoalveolar lavage fluid (BALF) and lung tissues were evaluated. Results: In lung tissues, airway remodeling occurred after 9 weeks of OVA sensitization. Peak levels of anti-elastin Ab and eosinophils in BALF were detected after 3 weeks of OVA sensitization. Anti-elastin Ab and eosinophil levels in BALF were significantly reduced after 3 weeks by the neutralization of anti-elastin Ab. Peak transforming growth factor-β1 levels in BALF were detected at 3 weeks after OVA sensitization and were significantly reduced by the neutralization of anti-elastin Ab. Airway remodeling in lung tissues was also significantly inhibited by the neutralization of anti-elastin Ab. Conclusions: In our murine asthma model, anti-elastin Ab was recruited to the airway by OVA-induced allergic inflammation. Airway remodeling was inhibited by the neutralization of anti-elastin Ab. Anti-elastin Ab may contribute to the progression of airway remodeling.
Databáze:	MEDLINE
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