Protein Localization in the Cyanobacterium Anabaena sp. PCC7120 Using Immunofluorescence Labeling.

Autor:	Trigo C; Laboratorio de Ecología Microbiana y Toxicología Ambiental, Department of Molecular Genetics and Microbiology, Pontificia Universidad Católica de Chile, Santiago, Chile., Andrade D; Laboratorio de Ecología Microbiana y Toxicología Ambiental, Department of Molecular Genetics and Microbiology, Pontificia Universidad Católica de Chile, Santiago, Chile., Vásquez M; Laboratorio de Ecología Microbiana y Toxicología Ambiental, Department of Molecular Genetics and Microbiology, Pontificia Universidad Católica de Chile, Santiago, Chile.
Jazyk:	angličtina
Zdroj:	Bio-protocol [Bio Protoc] 2017 Jun 05; Vol. 7 (11), pp. e2318. Date of Electronic Publication: 2017 Jun 05 (Print Publication: 2017).
DOI:	10.21769/BioProtoc.2318
Abstrakt:	Techniques such as immunoflorescence are widely used to determine subcellular distribution of proteins. Here we report on a method to immunolocalize proteins in Anabaena sp. PCC7120 with fluorophore-conjugated antibodies by fluorescence microscopy. This method improves the permeabilization of cyanobacterial cells and minimizes the background fluorescence for non-specific attachments. In this protocol, rabbit antibodies were raised against the synthetic peptide of CyDiv protein ( Mandakovic et al. , 2016 ). The secondary antibody conjugated to the fluorophore Alexa488 was used due to its different emission range in comparison to the autofluorescence of the cyanobacterium. (Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.)
Databáze:	MEDLINE
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