Venous puncture wound hemostasis results in a vaulted thrombus structured by locally nucleated platelet aggregates.

Autor: Rhee SW; Department of Pharmacology and Toxicology, University of Arkansas for Medical Sciences, Little Rock, AR, USA., Pokrovskaya ID; Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR, USA., Ball KK; Department of Pharmacology and Toxicology, University of Arkansas for Medical Sciences, Little Rock, AR, USA., Ling K; Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA., Vedanaparti Y; Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA., Cohen J; Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA., Cruz DRD; Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA., Zhao OS; Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA., Aronova MA; Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA., Zhang G; Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA., Kamykowski JA; Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR, USA., Leapman RD; Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA., Storrie B; Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR, USA. StorrieBrian@uams.edu.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2021 Sep 16; Vol. 4 (1), pp. 1090. Date of Electronic Publication: 2021 Sep 16.
DOI: 10.1038/s42003-021-02615-y
Abstrakt: Primary hemostasis results in a platelet-rich thrombus that has long been assumed to form a solid plug. Unexpectedly, our 3-dimensional (3D) electron microscopy of mouse jugular vein puncture wounds revealed that the resulting thrombi were structured about localized, nucleated platelet aggregates, pedestals and columns, that produced a vaulted thrombus capped by extravascular platelet adherence. Pedestal and column surfaces were lined by procoagulant platelets. Furthermore, early steps in thrombus assembly were sensitive to P2Y 12 inhibition and late steps to thrombin inhibition. Based on these results, we propose a Cap and Build, puncture wound paradigm that should have translational implications for bleeding control and hemostasis.
(© 2021. The Author(s).)
Databáze: MEDLINE
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