Modeling Human Genetic Disorders with CRISPR Technologies in Xenopus .
| Autor: | Willsey HR; Department of Psychiatry and Behavioral Sciences, Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, California 94143, USA; helen.willsey@ucsf.edu rmg9p@virginia.edu., Guille M; European Xenopus Resource Centre, School of Biological Sciences, University of Portsmouth, Portsmouth PO1 2UP, United Kingdom., Grainger RM; Department of Biology, University of Virginia, Charlottesville, Virginia 22904, USA helen.willsey@ucsf.edu rmg9p@virginia.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2022 Mar 01; Vol. 2022 (3). Date of Electronic Publication: 2022 Mar 01. |
| DOI: | 10.1101/pdb.prot106997 |
| Abstrakt: | Combining the power of Xenopus developmental biology with CRISPR-based technologies promises great discoveries in understanding and treating human genetic disorders. Here we provide a practical pipeline for how to go from known disease gene(s) or risk gene(s) of interest to methods for gaining functional insight into the contribution of these genes to disorder etiology in humans. (© 2022 Cold Spring Harbor Laboratory Press.) |
| Databáze: | MEDLINE |
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