Placental Passage of Humulone and Protopine in an Ex Vivo Human Perfusion System.

Autor:	Spiess D; Department of Obstetrics, University Hospital Zurich, University of Zurich, Zurich, Switzerland.; Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Basel, Switzerland., Abegg VF; Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Basel, Switzerland., Chauveau A; Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Basel, Switzerland., Treyer A; Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Basel, Switzerland., Reinehr M; Department of Pathology and Molecular Pathology, University Hospital Zurich, Zurich, Switzerland., Oufir M; Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Basel, Switzerland., Duong E; Department of Obstetrics, University Hospital Zurich, University of Zurich, Zurich, Switzerland.; Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Basel, Switzerland., Potterat O; Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Basel, Switzerland., Hamburger M; Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Basel, Switzerland., Simões-Wüst AP; Department of Obstetrics, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
Jazyk:	angličtina
Zdroj:	Planta medica [Planta Med] 2021 Nov; Vol. 87 (14), pp. 1192-1205. Date of Electronic Publication: 2021 Sep 16.
DOI:	10.1055/a-1578-3803
Abstrakt:	The placental passage of humulone and protopine was investigated with a human ex vivo placental perfusion model. The model was first validated with diazepam and citalopram, 2 compounds known to cross the placental barrier, and antipyrine as a positive control. All compounds were quantified by partially validated U(H)PLC-MS/MS bioanalytical methods. Only a small portion of humulone initially present in the maternal circuit reached the fetal circuit. The humulone concentration in the maternal circuit rapidly decreased, likely due to metabolization in the placenta. Protopine was transferred from the maternal to the fetal circuit, with a steady-state reached after 90 min. None of the study compounds affected placental viability or functionality, as glucose consumption, lactate production, beta-human chorionic gonadotropin, and leptin release remained constant. Histopathological evaluation of all placental specimens showed unremarkable, age-appropriate parenchymal maturation with no pathologic findings. Competing Interests: The authors declare that they have no conflict of interest. (The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).)
Databáze:	MEDLINE
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