WHotLAMP: A simple, inexpensive, and sensitive molecular test for the detection of SARS-CoV-2 in saliva.

Autor: Ng D; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, United States of America., Pinharanda A; Department of Biological Sciences, Columbia University, New York, NY, United States of America., Vogt MC; Department of Biological Sciences, Columbia University, New York, NY, United States of America.; Howard Hughes Medical Institute, Columbia University, New York, NY, United States of America., Litwin-Kumar A; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, United States of America., Stearns K; Department of Anesthesiology, Columbia University Irving Medical Center, New York, NY, United States of America., Thopte U; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, United States of America., Cannavo E; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, United States of America., Enikolopov A; Independent Researched, New York, NY, United States of America., Fiederling F; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, United States of America., Kosmidis S; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, United States of America., Noro B; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, United States of America., Rodrigues-Vaz I; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, United States of America., Shayya H; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, United States of America., Andolfatto P; Department of Biological Sciences, Columbia University, New York, NY, United States of America., Peterka DS; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, United States of America., Tabachnik T; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, United States of America., D'Armiento J; Department of Anesthesiology, Columbia University Irving Medical Center, New York, NY, United States of America.; Center for LAM and Rare Lung Diseases, Columbia University Irving Medical Center, New York, NY, United States of America., Goldklang M; Department of Anesthesiology, Columbia University Irving Medical Center, New York, NY, United States of America.; Center for LAM and Rare Lung Diseases, Columbia University Irving Medical Center, New York, NY, United States of America., Bendesky A; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, United States of America.; Department of Ecology, Evolution and Environmental Biology, Columbia University, New York, NY, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2021 Sep 16; Vol. 16 (9), pp. e0257464. Date of Electronic Publication: 2021 Sep 16 (Print Publication: 2021).
DOI: 10.1371/journal.pone.0257464
Abstrakt: Despite the development of effective vaccines against SARS-CoV-2, epidemiological control of the virus is still challenging due to slow vaccine rollouts, incomplete vaccine protection to current and emerging variants, and unwillingness to get vaccinated. Therefore, frequent testing of individuals to identify early SARS-CoV-2 infections, contact-tracing and isolation strategies remain crucial to mitigate viral spread. Here, we describe WHotLAMP, a rapid molecular test to detect SARS-CoV-2 in saliva. WHotLAMP is simple to use, highly sensitive (~4 viral particles per microliter of saliva) and specific, as well as inexpensive, making it ideal for frequent screening. Moreover, WHotLAMP does not require toxic chemicals or specialized equipment and thus can be performed in point-of-care settings, and may also be adapted for resource-limited environments or home use. While applied here to SARS-CoV-2, WHotLAMP can be modified to detect other pathogens, making it adaptable for other diagnostic assays, including for use in future outbreaks.
Competing Interests: Patent application 63/088,694 related to this technology was filed by Columbia University, to facilitate that this technology be made widely available. AB became a member of the advisory board of Rapid Diagnostic Systems Limited for which he received options, after the conclusion of experiments and data analyses described here. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
Databáze: MEDLINE
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