Multi-omic approach to evaluate the response of gilt-head sea bream (Sparus aurata) exposed to the UV filter sulisobenzone.

Autor: Colás-Ruiz NR; Faculty of Marine and Environmental Sciences (CASEM), University of Cadiz, 11510 Puerto Real, Cádiz, Spain., Ramirez G; Hydrosciences Montpellier, University of Montpellier, IRD, CNRS, Montpellier, France., Courant F; Hydrosciences Montpellier, University of Montpellier, IRD, CNRS, Montpellier, France., Gomez E; Hydrosciences Montpellier, University of Montpellier, IRD, CNRS, Montpellier, France., Hampel M; Faculty of Marine and Environmental Sciences (CASEM), University of Cadiz, 11510 Puerto Real, Cádiz, Spain., Lara-Martín PA; Faculty of Marine and Environmental Sciences (CASEM), University of Cadiz, 11510 Puerto Real, Cádiz, Spain. Electronic address: pablo.lara@uca.es.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2022 Jan 10; Vol. 803, pp. 150080. Date of Electronic Publication: 2021 Sep 03.
DOI: 10.1016/j.scitotenv.2021.150080
Abstrakt: Sulisobenzone (BP-4) is one of the benzophenone type UV filters most frequently detected in aquatic ecosystems. As a suspected endocrine disrupting compound, scarce information is available yet about other molecular effects and its mechanism of action. Here, we used an integrated transcriptomic and metabolomic approach to improve the current understanding on the toxicity of BP-4 towards aquatic species. Gilt-head sea bream individuals were exposed at environmentally relevant concentrations (10 μg L -1 ) for 22 days. Transcriptomic analysis revealed 371 differentially expressed genes in liver while metabolomic analysis identified 123 differentially modulated features in plasma and 118 in liver. Integration of transcriptomic and metabolomic data showed disruption of the energy metabolism (>10 pathways related to the metabolism of amino acids and carbohydrates were impacted) and lipid metabolism (5 glycerophospholipids and the expression of 3 enzymes were affected), suggesting oxidative stress. We also observed, for the first time in vivo and at environmental relevant concentrations, the disruption of several enzymes involved in the steroid and thyroid hormones biosynthesis. DNA and RNA synthesis was also impacted by changes in the purine and pyrimidine metabolisms. Overall, the multiomic workflow presented here increases the evidence on suspected effects of BP-4 exposure and identifies additional modes of action of the compounds that could have been overlooked by using single omic approaches.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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