Embryotoxic activity of 3C protease of human hepatitis A virus in developing Danio rerio embryos.

Autor: Selina PI; Institute of Molecular Genetics of National Research Center, Kurchatov Institute, 123182, Moscow, Russia. greenapple_35@mail.ru., Karaseva MA; Institute of Molecular Genetics of National Research Center, Kurchatov Institute, 123182, Moscow, Russia., Komissarov AA; Institute of Molecular Genetics of National Research Center, Kurchatov Institute, 123182, Moscow, Russia., Safina DR; Institute of Molecular Genetics of National Research Center, Kurchatov Institute, 123182, Moscow, Russia., Lunina NA; Institute of Molecular Genetics of National Research Center, Kurchatov Institute, 123182, Moscow, Russia., Roschina MP; Institute of Molecular Genetics of National Research Center, Kurchatov Institute, 123182, Moscow, Russia., Sverdlov ED; Institute of Molecular Genetics of National Research Center, Kurchatov Institute, 123182, Moscow, Russia., Demidyuk IV; Institute of Molecular Genetics of National Research Center, Kurchatov Institute, 123182, Moscow, Russia., Kostrov SV; Institute of Molecular Genetics of National Research Center, Kurchatov Institute, 123182, Moscow, Russia.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Sep 14; Vol. 11 (1), pp. 18196. Date of Electronic Publication: 2021 Sep 14.
DOI: 10.1038/s41598-021-97641-5
Abstrakt: The 3C protease is a key factor in picornavirus-induced pathologies with a comprehensive action on cell targets. However, the effects induced by the enzyme have not been described at the organismic level. Here, the model of developing Danio rerio embryos was used to analyze possible toxic effects of the 3C protease of human hepatitis A virus (3Cpro) at the whole-body level. The transient 3Cpro expression had a notable lethal effect and induced a number of specific abnormalities in Danio rerio embryos within 24 h. These effects are due to the proteolytic activity of the enzyme. At the same time, the 3Cpro variant with reduced catalytic activity (3Cmut) increased the incidence of embryonic abnormalities; however, this effect was smaller compared to the native enzyme form. While the expression of 3Cmut increased the overall rate of abnormalities, no predominance of specific ones was observed. The data obtained point to a presence significant impact of picornavirus 3Cprotease at the whole-organism level and make contribution to the study of the infectious process caused by human hepatitis A virus.
(© 2021. The Author(s).)
Databáze: MEDLINE
Externí odkaz: