Cancerous and non-neoplastic stem cells in the stomach similarly express CD44 and CD133.

Autor: Feitosa NPP; Graduation Program of Pathology, Department of Pathology and Forensic Medicine, Faculty of Medicine, Federal University of Ceara, 60441750 Fortaleza, Brazil., Pereira VBM; Drug Research and Development Center, Graduation Program in Pharmacology, Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceara, 60430275 Fortaleza, Brazil., Silva BGB; Graduation Program of Pathology, Department of Pathology and Forensic Medicine, Faculty of Medicine, Federal University of Ceara, 60441750 Fortaleza, Brazil., Queroz AVF; Graduation Program of Pathology, Department of Pathology and Forensic Medicine, Faculty of Medicine, Federal University of Ceara, 60441750 Fortaleza, Brazil., Rodrigues BJ; Graduation Program of Pathology, Department of Pathology and Forensic Medicine, Faculty of Medicine, Federal University of Ceara, 60441750 Fortaleza, Brazil., Costa MLV; Department of Surgery, Faculty of Medicine, Federal University of Ceara, 60430140 Fortaleza, Brazil., Alencar CH; Department of Community Health of the Faculty of Medicine of the Federal University of Ceara, 60430235 Fortaleza, Brazil., Lima-Júnior RCP; Drug Research and Development Center, Graduation Program in Pharmacology, Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceara, 60430275 Fortaleza, Brazil., Wong DVT; Graduation Program of Pathology, Department of Pathology and Forensic Medicine, Faculty of Medicine, Federal University of Ceara, 60441750 Fortaleza, Brazil., Frota CC; Graduation Program of Pathology, Department of Pathology and Forensic Medicine, Faculty of Medicine, Federal University of Ceara, 60441750 Fortaleza, Brazil., Almeida PRC; Graduation Program of Pathology, Department of Pathology and Forensic Medicine, Faculty of Medicine, Federal University of Ceara, 60441750 Fortaleza, Brazil. Electronic address: paulo.almeida@ufc.br.
Jazyk: angličtina
Zdroj: Acta histochemica [Acta Histochem] 2021 Oct; Vol. 123 (7), pp. 151787. Date of Electronic Publication: 2021 Sep 10.
DOI: 10.1016/j.acthis.2021.151787
Abstrakt: CD44 and CD133 have been considered as cancer stem cell (CSC) markers. Stem cell markers are rarely described in healthy stomach tissues. However, the clinicopathological and prognostic value of CD44 and CD133 in gastric cancer remains controversial. This study investigated the expression of CD44 and CD133 in gastric cancer and non-neoplastic gastric mucosa. We used samples of primary gastric adenocarcinomas (n = 69), metastatic lymph nodes (n = 30), intestinal metaplasia (n = 17), and histologically normal gastric tissues of surgical margins (n = 54). The expression of CD44 and CD133 were studied in samples by immunohistochemistry. Fisher's exact test and a logistic regression model were used in this study. CD44 expression was observed in 12% of samples with intestinal metaplasia, 20% with lymph node metastases, 22% with normal mucosa, to 30% of samples with primary tumors. Most of these positive tumors showed immunostaining in less than 4% of cancerous cells, mainly in the diffuse type. CD133 expression was observed in 7% (intestinal metaplasia) to 46% (normal mucosa). In the positive cases of cancer (24%), in most of them, less than 3% of cells were marked. CD44 and CD133 expression in the histologically normal gastric mucosa was restricted to the deeper regions of the gastric crypts at the level where stem cells and progenitor cells are usually found. CD44 and CD133 expression occurs in few gastric cancer cells, mainly in diffuse carcinomas, and are expressed in histologically normal gastric mucosae. None of the markers are specific for cancer and are also present in intestinal metaplasia and the normal mucosa.
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Databáze: MEDLINE
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