Interdependence of a mechanosensitive anion channel and glutamate receptors in distal wound signaling.

Autor: Moe-Lange J; Department of Biology, Stanford University, Stanford, CA 94305, USA.; Institute for Molecular Physiology, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany.; Department of Plant Biology, Carnegie Science, Stanford, CA 94305, USA., Gappel NM; Institute for Molecular Physiology, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany., Machado M; Department of Plant Biology, Carnegie Science, Stanford, CA 94305, USA., Wudick MM; Institute for Molecular Physiology, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany., Sies CSA; Institute for Molecular Physiology, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany., Schott-Verdugo SN; Institute for Pharmaceutical and Medicinal Chemistry, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany.; Centro de Bioinformática y Simulación Molecular (CBSM), Facultad de Ingeniería, Universidad de Talca, 2 Norte 685, CL-3460000 Talca, Chile.; John von Neumann Institute for Computing (NIC), Jülich Supercomputing Centre (JSC), Institute of Biological Information Processing (IBI-7: Structural Bioinformatics), and Institute of Bio- and Geosciences (IBG-4: Bioinformatics), Forschungszentrum Jülich GmbH, Wilhelm-Johnen-Str., 52425 Jülich, Germany., Bonus M; Institute for Pharmaceutical and Medicinal Chemistry, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany., Mishra S; Computational Cell Biology, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany., Hartwig T; Institute for Molecular Physiology, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany., Bezrutczyk M; Institute for Molecular Physiology, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany., Basu D; NSF Center for Engineering Mechanobiology, Department of Biology, Washington University in St. Louis, Box 1137, One Brookings Drive, St. Louis, MO 63130, USA., Farmer EE; Department of Plant Molecular Biology, University of Lausanne, CH-1015 Lausanne, Switzerland., Gohlke H; Institute for Pharmaceutical and Medicinal Chemistry, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany.; John von Neumann Institute for Computing (NIC), Jülich Supercomputing Centre (JSC), Institute of Biological Information Processing (IBI-7: Structural Bioinformatics), and Institute of Bio- and Geosciences (IBG-4: Bioinformatics), Forschungszentrum Jülich GmbH, Wilhelm-Johnen-Str., 52425 Jülich, Germany., Malkovskiy A; Department of Plant Biology, Carnegie Science, Stanford, CA 94305, USA., Haswell ES; NSF Center for Engineering Mechanobiology, Department of Biology, Washington University in St. Louis, Box 1137, One Brookings Drive, St. Louis, MO 63130, USA., Lercher MJ; Computational Cell Biology, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany., Ehrhardt DW; Department of Plant Biology, Carnegie Science, Stanford, CA 94305, USA., Frommer WB; Department of Biology, Stanford University, Stanford, CA 94305, USA.; Institute for Molecular Physiology, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany.; Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya 464-8601, Japan., Kleist TJ; Institute for Molecular Physiology, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany.
Jazyk: angličtina
Zdroj: Science advances [Sci Adv] 2021 Sep 10; Vol. 7 (37), pp. eabg4298. Date of Electronic Publication: 2021 Sep 08.
DOI: 10.1126/sciadv.abg4298
Abstrakt: Glutamate has dual roles in metabolism and signaling; thus, signaling functions must be isolatable and distinct from metabolic fluctuations, as seen in low-glutamate domains at synapses. In plants, wounding triggers electrical and calcium (Ca 2+ ) signaling, which involve homologs of mammalian glutamate receptors. The hydraulic dispersal and squeeze-cell hypotheses implicate pressure as a key component of systemic signaling. Here, we identify the stretch-activated anion channel MSL10 as necessary for proper wound-induced electrical and Ca 2+ signaling. Wound gene induction, genetics, and Ca 2+ imaging indicate that MSL10 acts in the same pathway as the glutamate receptor–like proteins (GLRs). Analogous to mammalian NMDA glutamate receptors, GLRs may serve as coincidence detectors gated by the combined requirement for ligand binding and membrane depolarization, here mediated by stretch activation of MSL10. This study provides a molecular genetic basis for a role of mechanical signal perception and the transmission of long-distance electrical and Ca 2+ signals in plants.
Databáze: MEDLINE
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