Cytotoxicity and dentin composition alterations promoted by different chemomechanical caries removal agents: A preliminary in vitro study.

Autor:	Guedes FR; Department of Pediatric Dentistry, School of Dentistry, Federal University of Uberlandia, Uberlandia, MG, Brazil., Bonvicini JF; Department of Pediatric Dentistry, School of Dentistry, Federal University of Uberlandia, Uberlandia, MG, Brazil., de Souza GL; Department of Endodontics, School of Dentistry, Federal University of Uberlandia, Uberlandia, MG, Brazil., da Silva WH; Department of Pediatric Dentistry, School of Dentistry, Federal University of Uberlandia, Uberlandia, MG, Brazil., Moura CC; Department of Endodontics, School of Dentistry, Federal University of Uberlandia, Uberlandia, MG, Brazil., Paranhos LR; Department of Community and Preventive Dentistry, School of Dentistry, Federal University of Uberlandia, Uberlandia, MG, Brazil., Turrioni AP; Department of Pediatric Dentistry, School of Dentistry, Federal University of Uberlandia, Uberlandia, MG, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of clinical and experimental dentistry [J Clin Exp Dent] 2021 Aug 01; Vol. 13 (8), pp. e826-e834. Date of Electronic Publication: 2021 Aug 01 (Print Publication: 2021).
DOI:	10.4317/jced.58208
Abstrakt:	Background: The use of chemomechanical agents for caries removal has been indicated as a non-invasive treatment option; however, their possible deleterious effects on the dental-pulp complex have been insufficiently studied. This study assessed the direct cytotoxicity of two chemomechanical caries removal agents (Brix 3000™ - BX and Papacarie Duo™ - PD) on pulp cells from deciduous teeth, as well as to assess the morphology and chemical compositions of the dentin surface after the application of these materials. Material and Methods: The cells were seeded (50,000 cells/cm²) in a culture medium (DMEM with 10% fetal bovine serum - FBS). After 24 hours, the BX and PD materials were added to 1:20, 1:100, and 1:1000 dilutions. Non-exposed cells were considered as the control group. The viability test (MTT), Trypan Blue assay (TB), and cell morphology (Scanning Electron Microscopy - SEM) were performed after 24 hours of agent application. For the SEM and chemical (energy-dispersive X-ray spectrometry - EDS) dentin evaluation, 0.3-mm-thick dentin discs were obtained and divided into control group (no treatment) and surfaces covered with 37% phosphoric acid, BX, or PD. Data were compared by one-way ANOVA and Tukey's test ( p <0.05). Results: Decreases in cell viability and numbers of viable cells were observed for both materials, at all dilutions, when compared with the control group ( p <0.05). The BX and PD materials did not cause visually perceptible changes, according to SEM, on the surfaces of dentin discs. The EDS analysis did not indicate a statistically significant difference in the levels of calcium (Ca) and phosphorus (P) between the materials and the control group ( p >0.05). Conclusions: Both materials showed cytotoxicity when in direct contact with the pulp cells from deciduous teeth, and the BX material presented lower cytotoxicity than the PD material. Moreover, both materials did not significantly change the dentin composition. Key words: Cell culture, cytotoxicity, dental pulp, papacarie, primary teeth. Competing Interests: Conflicts of interest The authors declare that they have no conflict of interests. (Copyright: © 2021 Medicina Oral S.L.)
Databáze:	MEDLINE
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