Non-diphtheriae Corynebacterium species are associated with decreased risk of pneumococcal colonization during infancy.

Autor: Kelly MS; Botswana-University of Pennsylvania Partnership, Gaborone, Botswana. matthew.kelly@duke.edu.; Division of Pediatric Infectious Diseases, Duke University, Durham, NC, USA. matthew.kelly@duke.edu., Plunkett C; Division of Pediatric Infectious Diseases, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA., Yu Y; Department of Mathematics, North Carolina State University, Raleigh, NC, USA., Aquino JN; Division of Pediatric Infectious Diseases, Duke University, Durham, NC, USA., Patel SM; Division of Pulmonary Allergy, and Critical Care Medicine, Duke University, Durham, NC, USA., Hurst JH; Division of Pediatric Infectious Diseases, Duke University, Durham, NC, USA., Young RR; Division of Pediatric Infectious Diseases, Duke University, Durham, NC, USA., Smieja M; Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada., Steenhoff AP; Botswana-University of Pennsylvania Partnership, Gaborone, Botswana.; Global Health Center, Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Division of Pediatric Infectious Diseases, Children's Hospital of Philadelphia, Philadelphia, PA, USA., Arscott-Mills T; Botswana-University of Pennsylvania Partnership, Gaborone, Botswana.; Global Health Center, Children's Hospital of Philadelphia, Philadelphia, PA, USA., Feemster KA; Division of Pediatric Infectious Diseases, Children's Hospital of Philadelphia, Philadelphia, PA, USA., Boiditswe S; Botswana-University of Pennsylvania Partnership, Gaborone, Botswana., Leburu T; Botswana-University of Pennsylvania Partnership, Gaborone, Botswana., Mazhani T; University of Botswana School of Medicine, Gaborone, Botswana., Patel MZ; University of Botswana School of Medicine, Gaborone, Botswana., Rawls JF; Department of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA., Jawahar J; Department of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA., Shah SS; Divisions of Hospital Medicine and Infectious Diseases, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA., Polage CR; Department of Pathology, Duke University, Durham, NC, USA., Cunningham CK; Division of Pediatric Infectious Diseases, Duke University, Durham, NC, USA., Seed PC; Division of Pediatric Infectious Diseases, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
Jazyk: angličtina
Zdroj: The ISME journal [ISME J] 2022 Mar; Vol. 16 (3), pp. 655-665. Date of Electronic Publication: 2021 Sep 11.
DOI: 10.1038/s41396-021-01108-4
Abstrakt: Streptococcus pneumoniae (pneumococcus) is a leading cause of severe infections among children and adults. Interactions between commensal microbes in the upper respiratory tract and S. pneumoniae are poorly described. In this study, we sought to identify interspecies interactions that modify the risk of S. pneumoniae colonization during infancy and to describe development of the upper respiratory microbiome during infancy in a sub-Saharan African setting. We collected nasopharyngeal swabs monthly (0-6 months of age) or bimonthly (6-12 months of age) from 179 mother-infant dyads in Botswana. We used 16S ribosomal RNA gene sequencing to characterize the nasopharyngeal microbiome and identified S. pneumoniae colonization using a species-specific PCR assay. We detect S. pneumoniae colonization in 144 (80%) infants at a median age of 71 days and identify a strong negative association between the relative abundance of the bacterial genera Corynebacterium within the infant nasopharyngeal microbiome and the risk of S. pneumoniae colonization. Using in vitro cultivation experiments, we demonstrate growth inhibition of S. pneumoniae by secreted factors from strains of several Corynebacterium species isolated from these infants. Finally, we demonstrate that antibiotic exposures and the winter season are associated with a decline in the relative abundance of Corynebacterium within the nasopharyngeal microbiome, while breastfeeding is associated with an increase in the Corynebacterium relative abundance. Our findings provide novel insights into the interspecies interactions that contribute to colonization resistance to S. pneumoniae and suggest that the nasopharyngeal microbiome may be a previously unrecognized mechanism by which environmental factors influence the risk of pneumococcal infections during childhood. Moreover, this work lays the foundation for future studies seeking to use targeted manipulation of the nasopharyngeal microbiome to prevent infections caused by S. pneumoniae.
(© 2021. The Author(s), under exclusive licence to International Society for Microbial Ecology.)
Databáze: MEDLINE
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