| Autor: |
Perovic Blagojevic IM; Department of Laboratory Diagnostic, Clinical Hospital Center, Dr Dragisa Misovic - Dedinje, Belgrade, Serbia., Vekic JZ; Department for Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia., Macut DP; Clinic of Endocrinology, Diabetes and Diseases of Metabolism, Clinical Center Serbia, Belgrade, Serbia.; Faculty of Medicine, University of Belgrade, Belgrade, Serbia., Ignjatovic SD; Department for Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.; Center for Medical Biochemistry, Clinical Center Serbia, Belgrade, Serbia., Miljkovic-Trailovic MM; Department for Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia., Zeljkovic AR; Department for Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia., Spasojevic-Kalimanovska VV; Department for Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia., Bozic-Antic IB; Clinic of Endocrinology, Diabetes and Diseases of Metabolism, Clinical Center Serbia, Belgrade, Serbia.; Faculty of Medicine, University of Belgrade, Belgrade, Serbia., Bjekic-Macut JD; Faculty of Medicine, University of Belgrade, Belgrade, Serbia.; University Medical Center, Bezanijska Kosa, Belgrade, Serbia., Kastratovic-Kotlica BA; Faculty of Medicine, University of Belgrade, Belgrade, Serbia.; Clinic of Obstetrics and Gynecology, Clinical Center Serbia, Belgrade, Serbia., Andric ZG; University Medical Center, Bezanijska Kosa, Belgrade, Serbia., Ilic DS; Clinic of Endocrinology, Diabetes and Diseases of Metabolism, Clinical Center Serbia, Belgrade, Serbia., Kotur-Stevuljevic JM; Department for Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia. |
| Abstrakt: |
Polycystic ovary syndrome (PCOS) is associated with altered lipid profile and increased small, dense LDL particles (sdLDL). Considering that paraoxonase 1 (PON1) is an antioxidative enzyme located on HDL particles, the aim of this study was to investigate the connection between oxidative stress (OS) and PON1 activity with lipoprotein subclasses in PCOS depending on obesity. In 115 PCOS patients, lipoprotein subclasses distributions were determined by gradient gel electrophoresis. OS status was assessed by total oxidative status (TOS), advanced oxidation protein products, malondialdehyde (MDA), prooxidant-antioxidant balance (PAB), total antioxidative status (TAS) and superoxide dismutase (SOD) and PON1 activity. Overweight/obese PCOS patients ( n 55) had increased OS compared with normal weight patients ( n 60). In addition, overweight/obese group had lower HDL size and higher proportion of HDL 3a subclasses ( P < 0·05). PAB was in negative correlation with HDL 2a ( P < 0·001), whereas MDA and SOD correlated positively with HDL 3 subclasses ( P < 0·05). Serum PON1 activity was positively associated with proportions of PON1 activity on HDL 2b ( P < 0·05) and 2a ( P < 0·01), but negatively with the proportion on HDL 3 particles ( P < 0·01). LDL B phenotype patients had increased TAS, SOD and PON1 activity on HDL 2b, but decreased PON1 activity on HDL 3 subclasses. OS is associated with altered lipoprotein subclasses distribution in PCOS patients. Obesity in PCOS affects the profile of HDL subclasses, reflected through the reduced proportion of PON1 activity on HDL 3 subclasses in the presence of sdLDL particles. |
