Regenerative effect of platelet-rich plasma in the murine ischemic limbs.

Autor: Stilhano RS; Center for Gene Therapy Investigation, Universidade Federal de São Paulo (UNIFESP), Brazil; Department of Physiological Sciences, Faculdade de Ciências Médicas da Santa Casa de São Paulo (FCMSCSP), Brazil., Denapoli PMA; Center for Gene Therapy Investigation, Universidade Federal de São Paulo (UNIFESP), Brazil., Gallo CC; Center for Gene Therapy Investigation, Universidade Federal de São Paulo (UNIFESP), Brazil., Samoto VY; Center for Gene Therapy Investigation, Universidade Federal de São Paulo (UNIFESP), Brazil., Ingham SJM; Department of Orthopedic Surgery, Universidade Federal de Sao Paulo, Sao Paulo, SP, Brazil., Abdalla RJ; Department of Orthopedic Surgery, Universidade Federal de Sao Paulo, Sao Paulo, SP, Brazil., Koh TJ; Department of Kinesiology and Nutrition, University of Illinois at Chicago, Chicago, United States of America., Han SW; Center for Gene Therapy Investigation, Universidade Federal de São Paulo (UNIFESP), Brazil; Department of Biophysics, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, SP, Brazil. Electronic address: sang.han@unifesp.br.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2021 Nov 01; Vol. 284, pp. 119934. Date of Electronic Publication: 2021 Sep 08.
DOI: 10.1016/j.lfs.2021.119934
Abstrakt: Aims: The purpose of this study was to investigate the effect of PPRP (pure PRP) and LPRP (PRP with leukocytes) on recovery from limb ischemia and on expression of growth factors involved in angiogenesis, myogenesis and fibrogenesis.
Material and Methods: PPRP and LPRP prepared by centrifugation were added to cultures of C2C12 and NIH3T3 cells (1 or 10% PRPs) to evaluate alterations in cell metabolism and expression of growth factors by MTT, ELISA and RT-qPCR, respectively. To evaluate in vivo regenerative effects, PRPs were injected into the ischemic limbs of BALB/c mice and muscle mass/strength and histomorphometry were evaluated after 30 days.
Key Findings: Mice treated with PRPs after limb ischemia showed an increase in the size of myofibers and muscle strength, reduced fibrosis and adipocytes, and decreased capillary density and necrosis scores compared to untreated mice. In cell culture, serum deprivation reduced the viability of C2C12 and NIH3T3 cells to about 50%, but the addition of 1% PRPs completely recovered this loss. Both PRPs, downregulated most of the tested genes; however, angiogenic gene Vegfa in C2C12 and the fibrogenic genes Col1a1 and Col3a1 in NIH3T3 cells were upregulated by LPRP.
Significance: PPRP and LPRP had similar effects in regulation of genes involved in angiogenesis, myogenesis and fibrogenesis. However, the presence of leucocytes did not significantly affect regenerative activities of PRP in the ischemic limb.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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