Functional basis of electron transport within photosynthetic complex I.

Autor: Richardson KH; School of Biological and Chemical Sciences, Queen Mary University of London, London, UK.; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, London, UK., Wright JJ; School of Biological and Chemical Sciences, Queen Mary University of London, London, UK.; Medical Research Council Mitochondrial Biology Unit, Wellcome Trust/MRC Building, Cambridge, UK., Šimėnas M; London Centre for Nanotechnology, University College London, London, UK., Thiemann J; Plant Biochemistry, Faculty of Biology and Biotechnology, Ruhr University Bochum, Bochum, Germany., Esteves AM; School of Biological and Chemical Sciences, Queen Mary University of London, London, UK., McGuire G; School of Biological and Chemical Sciences, Queen Mary University of London, London, UK.; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, London, UK., Myers WK; Inorganic Chemistry, University of Oxford, Oxford, UK., Morton JJL; London Centre for Nanotechnology, University College London, London, UK.; Department of Electronic & Electrical Engineering, UCL, London, UK., Hippler M; Institute of Plant Biology and Biotechnology, University of Münster, Münster, Germany.; Institute of Plant Science and Resources, Okayama University, Kurashiki, Japan., Nowaczyk MM; Plant Biochemistry, Faculty of Biology and Biotechnology, Ruhr University Bochum, Bochum, Germany., Hanke GT; School of Biological and Chemical Sciences, Queen Mary University of London, London, UK. g.hanke@qmul.ac.uk., Roessler MM; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, London, UK. m.roessler@imperial.ac.uk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 Sep 10; Vol. 12 (1), pp. 5387. Date of Electronic Publication: 2021 Sep 10.
DOI: 10.1038/s41467-021-25527-1
Abstrakt: Photosynthesis and respiration rely upon a proton gradient to produce ATP. In photosynthesis, the Respiratory Complex I homologue, Photosynthetic Complex I (PS-CI) is proposed to couple ferredoxin oxidation and plastoquinone reduction to proton pumping across thylakoid membranes. However, little is known about the PS-CI molecular mechanism and attempts to understand its function have previously been frustrated by its large size and high lability. Here, we overcome these challenges by pushing the limits in sample size and spectroscopic sensitivity, to determine arguably the most important property of any electron transport enzyme - the reduction potentials of its cofactors, in this case the iron-sulphur clusters of PS-CI (N0, N1 and N2), and unambiguously assign them to the structure using double electron-electron resonance. We have thus determined the bioenergetics of the electron transfer relay and provide insight into the mechanism of PS-CI, laying the foundations for understanding of how this important bioenergetic complex functions.
(© 2021. The Author(s).)
Databáze: MEDLINE
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