Thymoquinone ameliorates age-related hearing loss in C57BL/6J mice by modulating Sirt1 activity and Bak1 expression.

Autor: Salam SA; Department of Zoology, Faculty of Science, Alexandria University, Alexandria 21511, Egypt. Electronic address: sherine.abdel.salam@alexu.edu.eg., Mostafa F; Department of Zoology, Faculty of Science, Alexandria University, Alexandria 21511, Egypt. Electronic address: fatma.fars33@gmail.com., Alnamshan MM; Department of Biology, College of Science, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia. Electronic address: malnamshan@iau.edu.sa., Elshewemi SS; Department of Zoology, Faculty of Science, Alexandria University, Alexandria 21511, Egypt. Electronic address: salma_shewemi@yahoo.com., Sorour JM; Department of Zoology, Faculty of Science, Alexandria University, Alexandria 21511, Egypt. Electronic address: jehan.sorour@alexu.edu.eg.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2021 Nov; Vol. 143, pp. 112149. Date of Electronic Publication: 2021 Sep 08.
DOI: 10.1016/j.biopha.2021.112149
Abstrakt: Age-related hearing loss (AHL) is the most common sensory disorder of aged population. Currently, one of the most important sources of experimental medicine for AHL is medicinal plants. This study performed the first investigation of the effect of thymoquinone (TQ), a potent antioxidant, on AHL. Here, we used inbred C57BL/6J mice (B6 mice) as a successful experimental model of the early onset of AHL. The behavioral assessment of hearing revealed that the injection of a high dose of TQ (40 mg/kg; TQ 40 ) significantly improved the auditory sensitivity of B6 mice at all tested frequencies (8, 16 and 22 kHz). Histological sections of cochlea from B6 mice injected with a low dose (20 mg/kg; TQ 20 ) and high dose showed relatively less degenerative signs in the modiolus, hair cells and spiral ligaments, the main constituents of the cochlea. In addition, TQ 40 completely restored the normal pattern of hair cells in B6 mice, as shown in scanning electron micrographs. Our data indicated that TQ 20 and TQ 40 reduced levels of Bak1-mediated apoptosis in the cochlea of B6 mice. Interestingly, the level of Sirt1, a positive regulator of autophagy, was significantly increased in B6 mice administered TQ 40 . In conclusion, TQ relieves the symptoms of AHL by downregulating Bak1 and activating Sirt1 in the cochlea of B6 mice.
(Copyright © 2021 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
Databáze: MEDLINE
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