Differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens.

Autor: Hul LM; Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Centro-Oeste, Guarapuava, PR, 85040-080, Brazil., Ibelli AMG; Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Centro-Oeste, Guarapuava, PR, 85040-080, Brazil.; Embrapa Suínos e Aves, Concórdia, SC, 89715-899, Brazil., Savoldi IR; Programa de Pós-Graduação em Zootecnia, Centro de Educação Superior do Oeste (CEO), Universidade do Estado de Santa Catarina, UDESC, Chapecó, SC, 89815-630, Brazil., Marcelino DEP; Faculdade de Concórdia FACC, Concórdia, SC, 89701-130, Brazil., Fernandes LT; Embrapa Suínos e Aves, Concórdia, SC, 89715-899, Brazil., Peixoto JO; Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Centro-Oeste, Guarapuava, PR, 85040-080, Brazil.; Embrapa Suínos e Aves, Concórdia, SC, 89715-899, Brazil., Cantão ME; Embrapa Suínos e Aves, Concórdia, SC, 89715-899, Brazil., Higa RH; Embrapa Informática Agropecuária, Campinas, SP, 70770-901, Brazil., Giachetto PF; Embrapa Informática Agropecuária, Campinas, SP, 70770-901, Brazil., Coutinho LL; Departamento de Zootecnia, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba, SP, 13418-900, Brazil., Ledur MC; Embrapa Suínos e Aves, Concórdia, SC, 89715-899, Brazil. monica.ledur@embrapa.br.; Programa de Pós-Graduação em Zootecnia, Centro de Educação Superior do Oeste (CEO), Universidade do Estado de Santa Catarina, UDESC, Chapecó, SC, 89815-630, Brazil. monica.ledur@embrapa.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Sep 09; Vol. 11 (1), pp. 17965. Date of Electronic Publication: 2021 Sep 09.
DOI: 10.1038/s41598-021-97306-3
Abstrakt: Locomotor problems are among one of the main concerns in the current poultry industry, causing major economic losses and affecting animal welfare. The most common bone anomalies in the femur are dyschondroplasia, femoral head separation (FHS), and bacterial chondronecrosis with osteomyelitis (BCO), also known as femoral head necrosis (FHN). The present study aimed to identify differentially expressed (DE) genes in the articular cartilage (AC) of normal and FHS-affected broilers by RNA-Seq analysis. In the transcriptome analysis, 12,169 genes were expressed in the femur AC. Of those, 107 genes were DE (FDR < 0.05) between normal and affected chickens, of which 9 were downregulated and 98 were upregulated in the affected broilers. In the gene-set enrichment analysis using the DE genes, 79 biological processes (BP) were identified and were grouped into 12 superclusters. The main BP found were involved in the response to biotic stimulus, gas transport, cellular activation, carbohydrate-derived catabolism, multi-organism regulation, immune system, muscle contraction, multi-organism process, cytolysis, leukocytes and cell adhesion. In this study, the first transcriptome analysis of the broilers femur articular cartilage was performed, and a set of candidate genes (AvBD1, AvBD2, ANK1, EPX, ADA, RHAG) that could trigger changes in the broiler´s femoral growth plate was identified. Moreover, these results could be helpful to better understand FHN in chickens and possibly in humans.
(© 2021. The Author(s).)
Databáze: MEDLINE
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