Analysis of Patient Factors Affecting In Vitro Characteristics of Subacromial Bursal Connective Tissue Progenitor Cells during Rotator Cuff Repair.

Autor: Morikawa D; Department of Orthopaedic Surgery, University of Connecticut, Farmington, CT 06032, USA.; Department of Orthopaedic Surgery, Juntendo University Urayasu Hospital, Urayasu 279-0021, Japan., Hawthorne BC; Department of Orthopaedic Surgery, University of Connecticut, Farmington, CT 06032, USA., McCarthy MBR; Department of Orthopaedic Surgery, University of Connecticut, Farmington, CT 06032, USA., Bellas N; Department of Orthopaedic Surgery, University of Connecticut, Farmington, CT 06032, USA., Johnson JD; Department of Orthopaedic Surgery, University of Connecticut, Farmington, CT 06032, USA., Trudeau MT; Department of Orthopaedic Surgery, University of Connecticut, Farmington, CT 06032, USA., Murphy KV; Department of Orthopaedic Surgery, University of Connecticut, Farmington, CT 06032, USA., Mancini MR; Department of Orthopaedic Surgery, University of Connecticut, Farmington, CT 06032, USA., LeVasseur MR; Department of Orthopaedic Surgery, University of Connecticut, Farmington, CT 06032, USA., Cote MP; Department of Orthopaedic Surgery, University of Connecticut, Farmington, CT 06032, USA., Mazzocca AD; Department of Orthopaedic Surgery, University of Connecticut, Farmington, CT 06032, USA.
Jazyk: angličtina
Zdroj: Journal of clinical medicine [J Clin Med] 2021 Sep 04; Vol. 10 (17). Date of Electronic Publication: 2021 Sep 04.
DOI: 10.3390/jcm10174006
Abstrakt: Unsatisfactory failure rates following rotator cuff (RC) repair have led orthopaedic surgeons to explore biological augmentation of the healing enthesis. The subacromial bursa (SB) contains abundant connective tissue progenitor cells (CTPs) that may aid in this process. The purpose of the study was to investigate the influence of patient demographics and tear characteristics on the number of colony-forming units (CFUs) and nucleated cell count (NCC) of SB-derived CTPs. In this study, we harvested SB tissue over the supraspinatus tendon and muscle in 19 patients during arthroscopic RC repair. NCC of each sample was analyzed on the day of the procedure. After 14 days, CFUs were evaluated under a microscope. Spearman's rank correlation coefficient was then used to determine the relationship between CFUs or NCC and patient demographics or tear characteristics. The study found no significant correlation between patient demographics and the number of CFUs or NCC of CTPs derived from the SB ( p > 0.05). The study did significantly observe that increased tear size was negatively correlated with the number of CFUs ( p < 0.05). These results indicated that increased tear size, but not patient demographics, may influence the viability of CTPs and should be considered when augmenting RCrepairs with SB.
Databáze: MEDLINE
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