| Autor: |
Ivanova DI; Institute of Chemical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria., Nedialkov PT; Faculty of Pharmacy, Medical University, 1000 Sofia, Bulgaria., Tashev AN; Department of Dendrology, University of Forestry, 1756 Sofia, Bulgaria., Olech M; Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Lublin, 20-059 Lublin, Poland., Nowak R; Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Lublin, 20-059 Lublin, Poland., Ilieva YE; Faculty of Pharmacy, Medical University, 1000 Sofia, Bulgaria.; The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria., Kokanova-Nedialkova ZK; Faculty of Pharmacy, Medical University, 1000 Sofia, Bulgaria., Atanasova TN; Faculty of Pharmacy, Medical University, 1000 Sofia, Bulgaria., Angelov G; Institute of Chemical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria., Najdenski HM; The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria. |
| Abstrakt: |
Juniper representatives are natural sources of plenty of bioactive metabolites and have been used since ancient times as folk remedies against tapeworms, warts, cancer, etc. The antiproliferative activities of junipers are attributed to podophyllotoxin (PPT), which is a precursor for the synthesis of efficient anticancer drugs. However, the natural sources of PPT, Sinopodophyllum hexandrum (Royle) T. S. Ying and Podophyllum peltatum L., are already endangered species because of their intensive industrial exploitation. Therefore, identification of other sources of PPT is necessary. This study is a broad comparative investigation of junipers, for which original sources have been accessed from different continents of the world. The present research is aimed at the identification of species, producing PPT and other lignans at concentrations that are sufficient for the high antiproliferative activity of the corresponding extracts. Cytotoxic juniper leaf extracts demonstrated a broad spectrum of activity on a panel of cancer cell lines. The antiproliferative properties of junipers were attributed to the combined activity of great diversity of lignans (podophyllotoxin, deoxypodophyllotoxin, β-peltatin, yatein, matairesinol, anhydropodorhizol, etc.), detected by UHPLC-HRMS and LC-ESI-MS/MS in the corresponding extracts. Several species of the genus Juniperus L. were outlined as perspective sources of drug precursors with potential pharmaceutical applications. |
