Cadmium interference with iron sensing reveals transcriptional programs sensitive and insensitive to reactive oxygen species.

Autor:	McInturf SA; Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA., Khan MA; Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA., Gokul A; Environmental Biotechnology Laboratory, Department of Biotechnology, University of the Western Cape, Bellville, Cape Town, 7535, South Africa., Castro-Guerrero NA; Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA., Höhner R; School of Biological Sciences, Washington State University, PO Box 644236, Pullman, WA 99164-4236, USA., Li J; Department of Entomology and Plant Pathology, 217 Plant Sciences Building, University of Arkansas System Division of Agriculture, Fayetteville, AR 72701, USA., Marjault HB; Institute of Life Science, The Hebrew University of Jerusalem, Jerusalem, 91904Israel., Fichman Y; Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA., Kunz HH; School of Biological Sciences, Washington State University, PO Box 644236, Pullman, WA 99164-4236, USA.; Biozentrum der LMU München, Germany., Goggin FL; Department of Entomology and Plant Pathology, 217 Plant Sciences Building, University of Arkansas System Division of Agriculture, Fayetteville, AR 72701, USA., Keyster M; Environmental Biotechnology Laboratory, Department of Biotechnology, University of the Western Cape, Bellville, Cape Town, 7535, South Africa., Nechushtai R; Institute of Life Science, The Hebrew University of Jerusalem, Jerusalem, 91904Israel., Mittler R; Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA., Mendoza-Cózatl DG; Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA.; Environmental Biotechnology Laboratory, Department of Biotechnology, University of the Western Cape, Bellville, Cape Town, 7535, South Africa.
Jazyk:	angličtina
Zdroj:	Journal of experimental botany [J Exp Bot] 2022 Jan 05; Vol. 73 (1), pp. 324-338.
DOI:	10.1093/jxb/erab393
Abstrakt:	Iron (Fe) is an essential micronutrient whose uptake is tightly regulated to prevent either deficiency or toxicity. Cadmium (Cd) is a non-essential element that induces both Fe deficiency and toxicity; however, the mechanisms behind these Fe/Cd-induced responses are still elusive. Here we explored Cd- and Fe-associated responses in wild-type Arabidopsis and in a mutant that overaccumulates Fe (opt3-2). Gene expression profiling revealed a large overlap between transcripts induced by Fe deficiency and Cd exposure. Interestingly, the use of opt3-2 allowed us to identify additional gene clusters originally induced by Cd in the wild type but repressed in the opt3-2 background. Based on the high levels of H2O2 found in opt3-2, we propose a model where reactive oxygen species prevent the induction of genes that are induced in the wild type by either Fe deficiency or Cd. Interestingly, a defined cluster of Fe-responsive genes was found to be insensitive to this negative feedback, suggesting that their induction by Cd is more likely to be the result of an impaired Fe sensing. Overall, our data suggest that Fe deficiency responses are governed by multiple inputs and that a hierarchical regulation of Fe homeostasis prevents the induction of specific networks when Fe and H2O2 levels are elevated. (© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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