| Autor: |
Yakovlev IA; Nikolaev Institute of Inorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, 3 Acad. Lavrentiev Avenue, Novosibirsk 630090, Russia. amikhailov@niic.nsc.ru., Mikhailov AA; Nikolaev Institute of Inorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, 3 Acad. Lavrentiev Avenue, Novosibirsk 630090, Russia. amikhailov@niic.nsc.ru., Eremina JA; Nikolaev Institute of Inorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, 3 Acad. Lavrentiev Avenue, Novosibirsk 630090, Russia. amikhailov@niic.nsc.ru., Klyushova LS; Institute of Molecular Biology and Biophysics - Subdivision of FRC FTM, 2/12 Timakova str., Novosibirsk, 630060, Russia., Nadolinny VA; Nikolaev Institute of Inorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, 3 Acad. Lavrentiev Avenue, Novosibirsk 630090, Russia. amikhailov@niic.nsc.ru., Kostin GA; Nikolaev Institute of Inorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, 3 Acad. Lavrentiev Avenue, Novosibirsk 630090, Russia. amikhailov@niic.nsc.ru. |
| Abstrakt: |
The synthetic approaches for the preparation of trans (NO,OH)- cis (NO 2 ,NO 2 )-[RuNO(L) 2 (NO 2 ) 2 OH], where L = ethyl nicotinate (I) and methyl nicotinate (II), are reported. The structures of the complexes are characterized by X-ray diffraction and analyzed by Hirshfeld surface analysis. Both compounds show a nitric oxide release reaction under 445 or 532 nm irradiation of dimethyl sulfoxide (DMSO) solutions, which is studied by combined ultraviolet-visible- (UV-vis), infrared- (IR), and electron paramagnetic resonance (EPR) spectroscopy and density functional theory (DFT) calculations. The charge transfer from the OH-Ru-NO chain and nitrite ligands to the antibonding orbitals of Ru-NO is responsible for the photo-cleavage of the ruthenium-nitrosyl bond. The elimination of NO leads to a side reaction, namely the protonation of the parent hydroxyl compound. The cytotoxicity and photo-induced cytotoxicity investigations of both compounds on the breast adenocarcinoma cell line MCF-7 reveal that (I) and (II) are cytotoxic with IC 50 values of 27.5 ± 2.8 μM and 23.3 ± 0.3 μM, respectively. Moreover, (I) shows an increase of the toxicity after light irradiation by 7 times (IC 50 = 4.1 ± 0.1), which makes it a prominent target for deeper biological investigations. |
