Photoreceptor nanotubes mediate the in vivo exchange of intracellular material.

Autor: Ortin-Martinez A; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, Canada., Yan NE; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada., Tsai ELS; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, Canada., Comanita L; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, Canada., Gurdita A; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada., Tachibana N; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, Canada., Liu ZC; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, Canada., Lu S; Lunenfeld Tanenbaum Research Institute, Mount Sinai Hospital, Sinai Health Systems, Toronto, ON, Canada., Dolati P; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada., Pokrajac NT; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada., El-Sehemy A; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada., Nickerson PEB; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, Canada., Schuurmans C; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.; Department of Biochemistry, University of Toronto, Toronto, ON, Canada.; Sunnybrook Research Institute, Toronto, ON, Canada.; Department of Ophthalmology and Vision Sciences, University of Toronto, Toronto, ON, Canada., Bremner R; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.; Lunenfeld Tanenbaum Research Institute, Mount Sinai Hospital, Sinai Health Systems, Toronto, ON, Canada.; Department of Ophthalmology and Vision Sciences, University of Toronto, Toronto, ON, Canada., Wallace VA; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.; Department of Ophthalmology and Vision Sciences, University of Toronto, Toronto, ON, Canada.
Jazyk: angličtina
Zdroj: The EMBO journal [EMBO J] 2021 Nov 15; Vol. 40 (22), pp. e107264. Date of Electronic Publication: 2021 Sep 08.
DOI: 10.15252/embj.2020107264
Abstrakt: Emerging evidence suggests that intracellular molecules and organelles transfer between cells during embryonic development, tissue homeostasis and disease. We and others recently showed that transplanted and host photoreceptors engage in bidirectional transfer of intracellular material in the recipient retina, a process termed material transfer (MT). We used cell transplantation, advanced tissue imaging approaches, genetic and pharmacologic interventions and primary cell culture to characterize and elucidate the mechanism of MT. We show that MT correlates with donor cell persistence and the accumulation of donor-derived proteins, mitochondria and transcripts in acceptor cells in vivo. MT requires cell contact in vitro and is associated with the formation of stable microtubule-containing protrusions, termed photoreceptor nanotubes ( Ph NTs), that connect donor and host cells in vivo and in vitro. Ph NTs mediate GFP transfer between connected cells in vitro. Furthermore, interfering with Ph NT outgrowth by targeting Rho GTPase-dependent actin remodelling inhibits MT in vivo. Collectively, our observations provide evidence for horizontal exchange of intracellular material via nanotube-like connections between neurons in vivo.
(© 2021 The Authors. Published under the terms of the CC BY 4.0 license.)
Databáze: MEDLINE
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