Generation of multiple human iPSC lines from peripheral blood mononuclear cells of two SLC2A3 deletion and two SLC2A3 duplication carriers.

Autor:	Ziegler GC; Division of Molecular Psychiatry, Center of Mental Health, University of Würzburg, Würzburg, Germany; Department of Psychiatry, Psychosomatics and Psychotherapy, University Hospital Würzburg, Würzburg, Germany., Radtke F; Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, University Hospital Würzburg, Würzburg, Germany., Vitale MR; Division of Molecular Psychiatry, Center of Mental Health, University of Würzburg, Würzburg, Germany; Laboratory of Psychiatric Neurobiology, Institute of Molecular Medicine, I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Preuße A; Division of Molecular Psychiatry, Center of Mental Health, University of Würzburg, Würzburg, Germany., Klopocki E; Institute of Human Genetics, Biocentre, University of Würzburg, Würzburg, Germany., Herms S; Institute of Human Genetics and Department of Genomics, Life and Brain Center, University of Bonn, Bonn, Germany; Human Genomics Research Group, Department of Biomedicine, University Hospital Basel, Basel, Switzerland., Lesch KP; Division of Molecular Psychiatry, Center of Mental Health, University of Würzburg, Würzburg, Germany; Laboratory of Psychiatric Neurobiology, Institute of Molecular Medicine, I.M. Sechenov First Moscow State Medical University, Moscow, Russia; Department of Psychiatry and Neurobiology, School for Mental Health and Neuroscience (MHeNs), Maastricht University, Maastricht, the Netherlands.
Jazyk:	angličtina
Zdroj:	Stem cell research [Stem Cell Res] 2021 Oct; Vol. 56, pp. 102526. Date of Electronic Publication: 2021 Sep 01.
DOI:	10.1016/j.scr.2021.102526
Abstrakt:	Copy number variants of SLC2A3, which encodes the glucose transporter GLUT3, are associated with several neuropsychiatric and cardiac diseases. Here, we report the successful reprogramming of peripheral blood mononuclear cells from two SLC2A3 duplication and two SLC2A3 deletion carriers and subsequent generation of two transgene-free iPSC clones per donor by Sendai viral transduction. All eight clones represent bona fide hiPSCs with high expression of pluripotency genes, ability to differentiate into cells of all three germ layers and normal karyotype. The generated cell lines will be helpful to enlighten the role of glucometabolic alterations in pathophysiological processes shared across organ boundaries. (Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu