The effect of fs800 on female egg production in Schistosoma mansoni.

Autor: Alwan SN; Departments of Biochemistry and Structural Biology, UT Health at San Antonio, San Antonio, TX 78229, USA. Electronic address: alwan@uthscsa.edu., LoVerde PT; Departments of Biochemistry and Structural Biology, UT Health at San Antonio, San Antonio, TX 78229, USA; Pathology and Laboratory Medicine, UT Health at San Antonio, San Antonio, TX 78229, USA.
Jazyk: angličtina
Zdroj: Molecular and biochemical parasitology [Mol Biochem Parasitol] 2021 Sep; Vol. 245, pp. 111412. Date of Electronic Publication: 2021 Sep 04.
DOI: 10.1016/j.molbiopara.2021.111412
Abstrakt: During schistosomiasis, the paired Schistosoma mansoni female produces about 300 eggs each day. These eggs are responsible for the clinical picture and the transmission of the disease. During female development and egg production, fs800 is expressed only in female vitelline cells. Blast search of fs800 did not show similarities with any published sequences by NCBI. We hypothesize that the product of this gene plays a role in S. mansoni egg production. By using RNA interference to knockdown fs800 and quantitative PCR to measure the gene expression in the female schistosomes, we were able to demonstrate that fs800 product is crucial for viable egg production, it has no effect on worm health or male-female pairing. Our data suggest fs800 inhibition as a potential target to prevent transmission and pathology of schistosomiasis.
(Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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