| Autor: |
Agronenko VA; Kavetsky Institute for Oncology Problems, AS Akr. SSR, Kiev., Belkin AL, Tibilova NN, Pendrak KA, Povzhitkova MS, Petrenko SV, Tsisarenko TA, Markova NA, Platonova OV, Ermolchuk ON |
| Jazyk: |
angličtina |
| Zdroj: |
Biomaterials, artificial cells, and artificial organs [Biomater Artif Cells Artif Organs] 1987; Vol. 15 (1), pp. 237-48. |
| DOI: |
10.3109/10731198709118524 |
| Abstrakt: |
Incubation of long-preserved donor blood erythrocytes (over 21 days) with rejuvenating solution Erythropifaden resulted in an increased ATP concentration to 2.03 +/- 0.34 mmol/l; increased 2,3-DPG to 3.89 +/- 0.51 mmol/l, and the number of erythrocyte discoid forms was raised to 30%. Further blood perfusion through SKN-D hemosorbent allowed to reduce the following adenine level to zero; riboxine by 90; citrate and lactate by 50%. The plasma K+ was decreased from 29.8 +/- 1.25 to 13.0 +/- 0.87 mmol/l, while blood pH rose from 6.675 +/- 0.018 to 7.310 +/- 0.028. At the end of the perfusion, the ATP level was seen to further increase to 2.53 +/- 0.28 mmol/l, and 2,3-DPG to 4.99 +/- 0.7 mmol/l. The indices of osmotic resistance and deformability of erythrocytes were normalized. The discocyte number reached 40%. The amount of red blood cell irreversible forms declined from 26 to 13% without any increment plasma free hemoglobin. The data obtained confirm the high efficacy of the combination of the rejuvenating and hemosorption procedures when applied to stored long-preserved blood. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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