ε 2 , ε 3 , and ε 4 variants of ApoE; rs2228570 (VDR), rs4588 and rs7041 (VDBP) polymorphisms in patients with multiple sclerosis: A case-control study in Turkish population.

Autor:	Gezmis H; Department of Medical Biology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey., Mayda Domac F; Department of Neurology, University of Health Sciences, Erenkoy Mental and Nervous Diseases Training and Research Hospital, Istanbul, Turkey., Ormeci B; Department of Neurology, Yeditepe University Hospital, Istanbul, Turkey., Uyanik H; Department of Neurology, Yeditepe University Hospital, Istanbul, Turkey., Doran T; Department of Medical Biology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey., Keles EC; Department of Biostatistics, Faculty of Medicine, Yeditepe University, Istanbul, Turkey., Kirac D; Department of Medical Biology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey.
Jazyk:	angličtina
Zdroj:	International journal of clinical practice [Int J Clin Pract] 2021 Nov; Vol. 75 (11), pp. e14801. Date of Electronic Publication: 2021 Sep 16.
DOI:	10.1111/ijcp.14801
Abstrakt:	Aim of the Study: Multiple sclerosis (MS) is a degenerative disease characterized by autoimmune demyelination in the central nervous system. Yet, underlined genetics or environmental markers are still controversial. The impact of vitamin D and cholesterol on disease activity has been phrased by many studies; however, the data available for the Turkish population are very limited. This study aimed to investigate the effect of vitamin D-related polymorphisms (VDBP and VDR) and cholesterol-related variants of ApoE on Turkish MS patients. Materials and Methods: Total DNAs were extracted from peripheral blood samples of 51 MS patients and 50 healthy volunteers. rs4588 and rs7041 polymorphisms of VDBP, rs2228570 of VDR, as well as ε2, ε3, and ε4 variants of ApoE, were investigated by RT-PCR. Biochemical parameters which thought to be associated with MS were also measured. Results were evaluated statistically. Results: Homozygous mutant genotype and G allele of rs2228570 in VDR, as well as heterozygous genotype of rs4588 in VDBP, were found statistically high in patients. Total cholesterol, triglyceride, and LDL-C levels were found significantly high, whereas HDL-C and vitamin D levels were low in patients. An association was found between rs4588 variation and high triglyceride levels. Similar correlations were found between ε2 genotype and low LDL-C level; ε3 genotype and higher LDL-C. Gender, triglyceride, HDL-C, and AA genotype in rs4588 had a significant effect on MS progression. Conclusion: The variations of rs2228570 and rs4588, vitamin D deficiency, and biological parameters related to cholesterol metabolism may be associated with MS risk. (© 2021 John Wiley & Sons Ltd.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu