Non-clinical repeated dose 28-day oral toxicity, reproductive toxicity and cytotoxicity studies of the polar fraction of Parkinsonia aculeata aerial parts extract.

Autor:	Menezes TM; Department of Physiology and Pharmacology, Federal University of Pernambuco, (UFPE), Av. Prof. Moraes Rego, 1235; Cidade Universitária, 50670-901 Recife - PE, Brazil., de Souza Franco E; Department of Veterinary Medicine, Brazilian University Center, (UNIBRA), R. Padre Inglês, 257 Boa Vista - Recife/PE - CEP: 50050-230 Recife - PE, Brazil., de Almeida Sousa Lima LC; Department of Physiology and Pharmacology, Federal University of Pernambuco, (UFPE), Av. Prof. Moraes Rego, 1235; Cidade Universitária, 50670-901 Recife - PE, Brazil., de Souza Pereira ÁM; Department of Physiology and Pharmacology, Federal University of Pernambuco, (UFPE), Av. Prof. Moraes Rego, 1235; Cidade Universitária, 50670-901 Recife - PE, Brazil., de Santos Lima LW; Department of Physiology and Pharmacology, Federal University of Pernambuco, (UFPE), Av. Prof. Moraes Rego, 1235; Cidade Universitária, 50670-901 Recife - PE, Brazil., Quixabeira CMT; Department of Physiology and Pharmacology, Federal University of Pernambuco, (UFPE), Av. Prof. Moraes Rego, 1235; Cidade Universitária, 50670-901 Recife - PE, Brazil., da Silva JF; Department of Biochemistry, Federal University of Pernambuco, (UFPE), Av. Prof. Moraes Rego, 1235; Cidade Universitária, 50670-901 Recife - PE, Brazil., Cahu TB; Department of Biochemistry, Federal University of Pernambuco, (UFPE), Av. Prof. Moraes Rego, 1235; Cidade Universitária, 50670-901 Recife - PE, Brazil., de Souza Bezerra R; Department of Biochemistry, Federal University of Pernambuco, (UFPE), Av. Prof. Moraes Rego, 1235; Cidade Universitária, 50670-901 Recife - PE, Brazil., Lira EC; Department of Physiology and Pharmacology, Federal University of Pernambuco, (UFPE), Av. Prof. Moraes Rego, 1235; Cidade Universitária, 50670-901 Recife - PE, Brazil., Militão GCG; Department of Physiology and Pharmacology, Federal University of Pernambuco, (UFPE), Av. Prof. Moraes Rego, 1235; Cidade Universitária, 50670-901 Recife - PE, Brazil., de Sousa Maia MB; Department of Physiology and Pharmacology, Federal University of Pernambuco, (UFPE), Av. Prof. Moraes Rego, 1235; Cidade Universitária, 50670-901 Recife - PE, Brazil.
Jazyk:	angličtina
Zdroj:	Toxicology research [Toxicol Res (Camb)] 2021 Jul 07; Vol. 10 (4), pp. 792-799. Date of Electronic Publication: 2021 Jul 07 (Print Publication: 2021).
DOI:	10.1093/toxres/tfab052
Abstrakt:	This study was aimed to evaluate toxicity in repeated doses for 28 days, reproductive toxicity and cytotoxicity of a polar fraction obtained from the hydroethanolic extract of Parkinsonia aculeata (PfrHEPA) in experimental models. To perform the toxicity test in repeated doses for 28 days, male and female Wistar rats were treated via orogastric for 28 days with PfrHEPA (35, 70 or 140 mg/kg) according to the guidelines established by the Organisation for Economic Co-operation and Development (OECD) number 407 (1995). For assessment, the impact of PfrHEPA on the reproductive output various parameters were measured, including maternal weight, no. of pregnant females, female fertility index (%), gestation lengthtime, implantation sites, litter size and placental index of test animals. The cytotoxicity of PfrHEPA was performed on the tumor lines NCI-H292 (human lung carcinoma), HL-60 (human promyelocytic leukemia) and HCT-116 (colorectal cancer). In the repeated dose toxicity test for 28 days, no mortality was observed in the male and female rats treated with PfrHEPA as well as morphological changes and biochemical and hematological parameters. In the reproductive toxicity test, no abnormalities were observed related to the toxicological parameters in both mothers and offspring. Regarding the cytotoxicity assay, the PfrHEPA fraction did not demonstrate significant cytotoxic effect on the cell lines analyzed. The present results suggest the use of PfrHEPA is safe and well tolerated in rats. Further studies are planned to identify and purify the active compounds for subsequent in vivo evaluation. (© The Author(s) 2021. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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