Study of anesthetics for euthanasia in rats and mice: A systematic review and meta-analysis on the impact upon biological outcomes (SAFE-RM).
| Autor: | Tobar Leitão SA; Postgraduate Program in Cardiology and Cardiovascular Science, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2400, 90035-903 Porto Alegre, RS, Brazil; Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350 - sala 12113, 90035-903 Porto Alegre, RS, Brazil., Soares DDS; Postgraduate Program in Cardiology and Cardiovascular Science, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2400, 90035-903 Porto Alegre, RS, Brazil; Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350 - sala 12113, 90035-903 Porto Alegre, RS, Brazil., Carvas Junior N; Department of Evidence-Based Health, UNIFESP, Rua Isabel Schmidt, 349, São Paulo 04743-030, Brazil., Zimmer R; Postgraduate Program in Medical Science, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2400, 90035-903 Porto Alegre, RS, Brazil., Ludwig NF; Postgraduate Program in Genetics and Molecular Biology, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, 91501-970 Porto Alegre, RS, Brazil., Andrades M; Postgraduate Program in Cardiology and Cardiovascular Science, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2400, 90035-903 Porto Alegre, RS, Brazil; Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350 - sala 12113, 90035-903 Porto Alegre, RS, Brazil. Electronic address: meandrades@hcpa.edu.br. |
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| Jazyk: | angličtina |
| Zdroj: | Life sciences [Life Sci] 2021 Nov 01; Vol. 284, pp. 119916. Date of Electronic Publication: 2021 Sep 01. |
| DOI: | 10.1016/j.lfs.2021.119916 |
| Abstrakt: | Aim: To summarize the knowledge on the effect of anesthetics employed right before euthanasia on biological outcomes. Data Source: A systematic review of the literature to find studies with isoflurane, ketamine, halothane, pentobarbital, or thiopental just before euthanasia of laboratory rats or mice. Study Selection: Controlled studies with quantitative data available. Data Extraction: The search, data extraction, and risk of bias (RoB) were performed independently by two reviewers using a structured form. For each outcome, an effect size (ES) was calculated relative to the control group. Meta-analysis was performed using robust variance meta-regression for hierarchical data structures, with adjustment for small samples. Data Synthesis: We included 20 studies with 407 biological outcomes (110 unique). RoB analysis indicated that 87.5% of the domains evaluated showed unclear risk, 2% high risk, and 10.5% low risk. The effect size for all anesthetics considered together was 0.99 (CI Conclusion: We showed that anesthetics interfere differently with the majority of the outcomes assessed. However, our data did not support the use of one anesthetic over others or even the killing without anesthetics. We conclude that outcomes cannot be compared among studies without considering the killing method. This protocol was registered at Prospero (CRD42019119520). Funding: There was no direct funding for this research. (Copyright © 2021 Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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