Virucidal and antiviral activities of pomegranate (Punica granatum) extract against the mosquito-borne Mayaro virus.
Autor: | Salles TS; Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. tiagosouzasalles@ufrj.br.; Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. tiagosouzasalles@ufrj.br., Meneses MDF; Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Caldas LA; National Institute of Science and Technology for Structural Biology and Bioimaging, INBEB, Rio de Janeiro, RJ, Brazil.; Laboratory of Cellular Ultrastructure Hertha Meyer, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Sá-Guimarães TE; Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., de Oliveira DM; Natural Products Research Institute, IPPN, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Ventura JA; Capixaba Institute of Research, Technical Assistance and Rural Extension, Espirito Santo, Vitoria, Brazil., Azevedo RC; Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Kuster RM; Natural Products Research Institute, IPPN, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Chemistry Department, Federal University of Espírito Santo, Vitoria, Espirito Santo, Brazil., Soares MR; Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Ferreira DF; Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; National Institute of Science and Technology for Structural Biology and Bioimaging, INBEB, Rio de Janeiro, RJ, Brazil.; Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC, USA. |
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Jazyk: | angličtina |
Zdroj: | Parasites & vectors [Parasit Vectors] 2021 Sep 03; Vol. 14 (1), pp. 443. Date of Electronic Publication: 2021 Sep 03. |
DOI: | 10.1186/s13071-021-04955-4 |
Abstrakt: | Background: The arthropod-borne Mayaro virus (MAYV) causes "Mayaro fever," a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical South America. Recently, MAYV has attracted attention due to its likely urbanization. There are currently no licensed drugs against most mosquito-transmitted viruses. Punica granatum (pomegranate) fruits cultivated in Brazil have been subjected to phytochemical investigation for the identification and isolation of antiviral compounds. In the present study, we explored the antiviral activity of pomegranate extracts in Vero cells infected with Mayaro virus. Methods: The ethanol extract and punicalagin of pomegranate were extracted solely from the shell and purified by chromatographic fractionation, and were chemically identified using spectroscopic techniques. The cytotoxicity of the purified compounds was measured by the dye uptake assay, while their antiviral activity was evaluated by a virus yield inhibition assay. Results: Pomegranate ethanol extract (CC Conclusions: The P. granatum extract is a promising source of antiviral compounds against the alphavirus MAYV and represents an excellent candidate for future studies with other enveloped RNA viruses. (© 2021. The Author(s).) |
Databáze: | MEDLINE |
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