Structural features of nucleosomes in interphase and metaphase chromosomes.
| Autor: | Arimura Y; Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065, USA. Electronic address: yarimura@rockefeller.edu., Shih RM; Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065, USA., Froom R; Laboratory of Molecular Biophysics, The Rockefeller University, New York, NY 10065, USA., Funabiki H; Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065, USA. Electronic address: funabih@rockefeller.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Molecular cell [Mol Cell] 2021 Nov 04; Vol. 81 (21), pp. 4377-4397.e12. Date of Electronic Publication: 2021 Sep 02. |
| DOI: | 10.1016/j.molcel.2021.08.010 |
| Abstrakt: | Structural heterogeneity of nucleosomes in functional chromosomes is unknown. Here, we devise the template-, reference- and selection-free (TRSF) cryo-EM pipeline to simultaneously reconstruct cryo-EM structures of protein complexes from interphase or metaphase chromosomes. The reconstructed interphase and metaphase nucleosome structures are on average indistinguishable from canonical nucleosome structures, despite DNA sequence heterogeneity, cell-cycle-specific posttranslational modifications, and interacting proteins. Nucleosome structures determined by a decoy-classifying method and structure variability analyses reveal the nucleosome structural variations in linker DNA, histone tails, and nucleosome core particle configurations, suggesting that the opening of linker DNA, which is correlated with H2A C-terminal tail positioning, is suppressed in chromosomes. High-resolution (3.4-3.5 Å) nucleosome structures indicate DNA-sequence-independent stabilization of superhelical locations ±0-1 and ±3.5-4.5. The linker histone H1.8 preferentially binds to metaphase chromatin, from which chromatosome cryo-EM structures with H1.8 at the on-dyad position are reconstituted. This study presents the structural characteristics of nucleosomes in chromosomes. Competing Interests: Declaration of interests H.F. is affiliated with Graduate School of Medical Sciences, Weill Cornell Medicine, and the Cell Biology Program at the Sloan Kettering Institute. The authors declare no competing interests. (Copyright © 2021 Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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