| Autor: |
Nel WJ; Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Private Bag X20, Pretoria, 0028 South Africa., de Beer ZW; Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Private Bag X20, Pretoria, 0028 South Africa., Wingfield MJ; Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Private Bag X20, Pretoria, 0028 South Africa., Poulsen M; Section for Ecology and Evolution, Department of Biology, University of Copenhagen, DK-2100 Copenhagen, Denmark., Aanen DK; Laboratory of Genetics, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands., Wingfield BD; Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Private Bag X20, Pretoria, 0028 South Africa., Duong TA; Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Private Bag X20, Pretoria, 0028 South Africa. |
| Abstrakt: |
The Ophiostomatales (Ascomycota) accommodates more than 300 species characterized by similar morphological adaptations to arthropod dispersal. Most species in this order are wood-inhabiting fungi associated with bark or ambrosia beetles. However, a smaller group of species occur in other niches such as in soil and Protea infructescences. Recent surveys of Termitomyces fungus gardens (fungus combs) of fungus-growing termites led to the discovery of characteristic ophiostomatalean-like fruiting structures. In this study, these ophiostomatalean-like fungi were identified using morphological characteristics, conventional molecular markers, and whole genome sequencing. In addition, the influence of the extracts derived from various parts of Termitomyces combs on the growth of these fungi in culture was considered. Based on phylogenomic analyses, two new genera ( Intubia and Chrysosphaeria ) were introduced to accommodate these ophiostomatalean species. Phylogenetic analyses revealed that the isolates resided in three well-supported lineages, and these were described as three new species ( Intubia macrotermitinarum, I. oerlemansii , and Chrysosphaeria jan-nelii ). Culture-based studies showed that these species do not depend on the Termitomyces comb material for growth. |
