Structure impact on photodynamic therapy and cellular contrasting functions of colloids constructed from dimeric Au(I) complex and hexamolybdenum clusters.

Autor: Faizullin BA; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov str., 420088 Kazan, Russian Federation; Kazan (Volga region) Federal University, 18 Kremlyovskaya str., 420008 Kazan, Russian Federation. Electronic address: bulat_fayzullin95@mail.ru., Strelnik ID; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov str., 420088 Kazan, Russian Federation., Dayanova IR; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov str., 420088 Kazan, Russian Federation., Gerasimova TP; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov str., 420088 Kazan, Russian Federation., Kholin KV; Kazan National Research Technical University named after A.N. Tupolev - KAI, 10 K. Marx str., 420111 Kazan, Russian Federation., Nizameev IR; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov str., 420088 Kazan, Russian Federation., Voloshina AD; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov str., 420088 Kazan, Russian Federation., Gubaidullin AT; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov str., 420088 Kazan, Russian Federation., Fedosimova SV; Kazan (Volga region) Federal University, 18 Kremlyovskaya str., 420008 Kazan, Russian Federation., Mikhailov MA; Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentiev Ave., 630090 Novosibirsk, Russian Federation., Sokolov MN; Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentiev Ave., 630090 Novosibirsk, Russian Federation., Sibgatullina GV; Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, 2/31 Lobachevski str., 420111 Kazan, Russian Federation., Samigullin DV; Kazan National Research Technical University named after A.N. Tupolev - KAI, 10 K. Marx str., 420111 Kazan, Russian Federation; Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, 2/31 Lobachevski str., 420111 Kazan, Russian Federation., Petrov KA; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov str., 420088 Kazan, Russian Federation., Karasik AA; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov str., 420088 Kazan, Russian Federation., Mustafina AR; Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov str., 420088 Kazan, Russian Federation.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2021 Sep; Vol. 128, pp. 112355. Date of Electronic Publication: 2021 Jul 31.
DOI: 10.1016/j.msec.2021.112355
Abstrakt: Electrostatically driven self-assembly of [Au 2 L 2 ] 2+ (L is cyclic PNNP ligand) with [{Mo 6 I 8 }(L') 6 ] 2- (L' = I - , CH 3 COO - ) in aqueous solutions is introduced as facile route for combination of therapeutic and cellular contrasting functions within heterometallic colloids (Mo 6 -Au 2 ). The nature of L' affects the size and aggregation behavior of crystalline Mo 6 -Au 2 aggregates, which in turn affect the luminescence of the cluster units incorporated into Mo 6 -Au 2 colloids. The spin trap facilitated electron spin resonance spectroscopy technique indicates that the level of ROS generated by Mo 6 -Au 2 colloids is also affected by their size. Both (L' = I - , CH 3 COO - ) Mo 6 -Au 2 colloids undergo cell internalization, which is enhanced by their assembly with poly-DL-lysine (PL) for L' = CH 3 COO - , but remains unchanged for L' = I - . The colloids PL-Mo 6 -Au 2 (L' = CH 3 COO - ) are visualized as huge crystalline aggregates both outside and inside the cell cytoplasm by confocal microscopy imaging of the incubated cells, while the smaller sized (30-50 nm) PL-Mo 6 -Au 2 (L' = I - ) efficiently stain the cell nuclei. Quantitative colocalization analysis of PL-Mo 6 -Au 2 (L' = CH 3 COO - ) in lysosomal compartments points to the fast endo-lysosomal escape of the colloids followed by their intracellular aggregation. The cytotoxicity of PL-Mo 6 -Au 2 differs from that of Mo 6 and Au 2 blocks, predominantly acting through apoptotic pathway. The photodynamic therapeutic effect of the PL-Mo 6 -Au 2 colloids on the cancer cells correlates with their intracellular trafficking and aggregation.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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