Artemia salina as an animal model for the preliminary evaluation of snake venom-induced toxicity.
| Autor: | Okumu MO; Department of Public Health, Pharmacology, and Toxicology, University of Nairobi, Kenya., Mbaria JM; Department of Public Health, Pharmacology, and Toxicology, University of Nairobi, Kenya., Gikunju JK; Department of Medical Laboratory Science, Jomo Kenyatta University of Agriculture and Technology, Kenya., Mbuthia PG; Department of Veterinary Pathology, Microbiology, and Parasitology, University of Nairobi, Kenya., Madadi VO; Department of Chemistry, University of Nairobi, Kenya., Ochola FO; Department of Pharmacology and Toxicology, Moi University, Kenya., Jepkorir MS; Centre for Traditional Medicine and Drug Research, Kenya Medical Research Institute, Kenya. |
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| Jazyk: | angličtina |
| Zdroj: | Toxicon: X [Toxicon X] 2021 Aug 19; Vol. 12, pp. 100082. Date of Electronic Publication: 2021 Aug 19 (Print Publication: 2021). |
| DOI: | 10.1016/j.toxcx.2021.100082 |
| Abstrakt: | Lethality and cytotoxicity assays of snake venoms and their neutralization by antivenom require many mice for the experiments. Recent developments have prompted researchers to seek alternative strategies that minimize the use of mice in line with Russel and Burch's 3Rs philosophy (Replacement, Reduction, and Refinement). Artemia salina is an animal model widely used for toxicity screening. However, its use in snake venom toxinology is limited by a lack of data. The present study compared the toxicity of venoms from Bitis arietans , Naja ashei, and Naja subfulva using mice and Artemia salina. In the Artemia salina test at 24 h and the dermonecrotic test in mice, the toxicity of the venoms was in the order Naja ashei ~ Naja subfulva > Bitis arietans . In the lethality test in mice, the toxicity of the venoms was in the order Naja subfulva > Naja ashei > Bitis arietans . These findings suggest that the toxicity of the venoms in Artemia salina and the dermonecrotic bioassay in mice have a similar trend but differ from the lethality test in mice. Therefore, it may be relevant to further explore the Artemia salina bioassay as a potential surrogate test of dermonecrosis in mice. Studies with more venoms may be needed to establish the correlation between the Artemia salina bioassay and the dermonecrotic assay in mice. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 The Author(s).) |
| Databáze: | MEDLINE |
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