Dynamics of the compartmentalized Streptomyces chromosome during metabolic differentiation.

Autor: Lioy VS; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France. virginia.lioy@i2bc.paris-saclay.fr., Lorenzi JN; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Najah S; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Poinsignon T; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Leh H; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Saulnier C; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Aigle B; Université de Lorraine, INRAE, DynAMic, Nancy, France., Lautru S; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Thibessard A; Université de Lorraine, INRAE, DynAMic, Nancy, France., Lespinet O; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Leblond P; Université de Lorraine, INRAE, DynAMic, Nancy, France., Jaszczyszyn Y; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Gorrichon K; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Varoquaux N; Université Grenoble Alpes, CNRS, Grenoble INP, TIMC-IMAG, Grenoble, France., Junier I; Université Grenoble Alpes, CNRS, Grenoble INP, TIMC-IMAG, Grenoble, France., Boccard F; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Pernodet JL; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Bury-Moné S; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France. stephanie.bury-mone@i2bc.paris-saclay.fr.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 Sep 01; Vol. 12 (1), pp. 5221. Date of Electronic Publication: 2021 Sep 01.
DOI: 10.1038/s41467-021-25462-1
Abstrakt: Bacteria of the genus Streptomyces are prolific producers of specialized metabolites, including antibiotics. The linear chromosome includes a central region harboring core genes, as well as extremities enriched in specialized metabolite biosynthetic gene clusters. Here, we show that chromosome structure in Streptomyces ambofaciens correlates with genetic compartmentalization during exponential phase. Conserved, large and highly transcribed genes form boundaries that segment the central part of the chromosome into domains, whereas the terminal ends tend to be transcriptionally quiescent compartments with different structural features. The onset of metabolic differentiation is accompanied by a rearrangement of chromosome architecture, from a rather 'open' to a 'closed' conformation, in which highly expressed specialized metabolite biosynthetic genes form new boundaries. Thus, our results indicate that the linear chromosome of S. ambofaciens is partitioned into structurally distinct entities, suggesting a link between chromosome folding, gene expression and genome evolution.
(© 2021. The Author(s).)
Databáze: MEDLINE
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