Phytochemical profiling and antioxidant potential of Daphne mucronata Royle and action against paracetamol-induced hepatotoxicity and nephrotoxicity in rabbits.
| Autor: | Nazir N; Department of Bio Chemistry, University of Malakand, Chakdara, Dir (Lower) 18800, Khyber Pakhtunkhwa, Pakistan., Muhammad J; Department of Botany, University of Malakand, Chakdara, Dir (Lower) 18800, Khyber Pakhtunkhwa, Pakistan., Ghaffar R; Department of Pharmacy, University of Malakand, Chakdara, Dir (Lower) 18800, Khyber Pakhtunkhwa, Pakistan., Nisar M; Department of Botany, University of Malakand, Chakdara, Dir (Lower) 18800, Khyber Pakhtunkhwa, Pakistan., Zahoor M; Department of Bio Chemistry, University of Malakand, Chakdara, Dir (Lower) 18800, Khyber Pakhtunkhwa, Pakistan., Uddin F; University of Engineering & Technology, Mardan, Khyber Pakhtunkhwa, Pakistan., Ullah R; Department of Pharmacognosy, (MAPPRC), College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia., Alotaibi A; Basic Science Department, College of Medicine, Princess Nourah bint Abdulrahman University. Riyadh 11671, Saudi Arabia. |
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| Jazyk: | angličtina |
| Zdroj: | Saudi journal of biological sciences [Saudi J Biol Sci] 2021 Sep; Vol. 28 (9), pp. 5290-5301. Date of Electronic Publication: 2021 May 26. |
| DOI: | 10.1016/j.sjbs.2021.05.051 |
| Abstrakt: | The paracetamol-induced injuries of liver and kidneys in animals are mostly used to screen out the hepato and nephroprotective effect of extract or other therapeutic agents. In the present study total phenolic and flavonoid contents, in vitro antioxidant, and in vivo hepato/nephroprotective (on paracetamol-induced intoxication in experimental rabbits) potentials of the Daphne mucronata leaves methanolic extract were determined. For the identification of possible phytochemicals, HPLC (high performance liquid chromatography) analysis was carried out and a total of eight phenolic compounds; malic acid, gallic acid, chlorogenic acid, epigallocatechin gallate, quercetin, morin, ellagic acid, and rutin were identified. D. mucronata extract at doses of 250 and 500 mg/kg body weight were given for eight days to paracetamol intoxicated rabbits and the observed results were compared with standard Silymarin. The level of liver enzymes like aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, serum triglyceride, serum cholesterol, serum bilirubin, and kidneys biomarkers like serum urea, uric acid, and creatinine, as well as lipid peroxidation malondialdehyde contents were increased while the antioxidant enzymes like reduced glutathione and total antioxidant capacity were decreased. Furthermore, histopathological analysis of the liver and kidney tissues of control and treated groups also confirmed the hepatoprotective and nephroprotective effect of the D. mucronata which was most probably due to its high antioxidant phenolic and flavonoid phytoconstituents. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 The Author(s).) |
| Databáze: | MEDLINE |
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