In vitro Regeneration of Hyssopus officinalis L. and Plant Genetic Similarity.

Autor: Bulavin IV; Federal State Funded Institution of Science 'The Labor Red Banner Order Nikita Botanical Gardens-National Scientific Center of the RAS', Yalta, Russia. labgennbs@yandex.ru., Ivanova NN; Federal State Funded Institution of Science 'The Labor Red Banner Order Nikita Botanical Gardens-National Scientific Center of the RAS', Yalta, Russia., Mitrofanova IV; Federal State Funded Institution of Science 'The Labor Red Banner Order Nikita Botanical Gardens-National Scientific Center of the RAS', Yalta, Russia.
Jazyk: angličtina
Zdroj: Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections [Dokl Biol Sci] 2021 Jul; Vol. 499 (1), pp. 109-112. Date of Electronic Publication: 2021 Aug 30.
DOI: 10.1134/S0012496621040013
Abstrakt: Biotechnological methods are an essential component of plant genetic resources investigation that allow both to preserve a rare natural plant and useful selection genotypes, especially essential oil bearing plants used in medicine, perfume, food, etc. In the clonal micropropagation in vitro, the key moment is to retain genetic stability of in vitro material. It is considered that plant regeneration in vitro from meristem or vegetative buds gives identical clones, but the effect of the medium growth regulators on the genetic stability of in vitro plant material is discussed. Therefore, the objective of our work was to evaluate the genetic similarity of ex situ and in vitro plants. Investigation was performed on Hyssopus officinalis L. cv. Nikitskiy Beliy (NBG selection). Regeneration from the shoot single-node segments on the modified Murashige and Skoog culture medium (MS) supplemented with 6-benzylaminopurine (BAP) in concentration 0.3-0.9 mg/L and 0.1 mg/L Indole-3-butyric acid (IBA) was carried out. It was established, that application of 0.5 mg/L BAP was sufficient for the organ formation and development without morphological deviations. Genetic analysis based on RAPD and ISSR-PCR had shown full genetic similarity of the investigated ex situ and in vitro plants.
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Databáze: MEDLINE
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