| Autor: |
Holmes HL; Physiology & Biomedical Engineering, Mayo Clinic., Stiller AA; Physiology & Biomedical Engineering, Mayo Clinic., Moore CJ; SonoVol, Inc., Gregory AV; Radiology, Mayo Clinic., Roos CM; Surgery, Mayo Clinic., Miller JD; Physiology & Biomedical Engineering, Mayo Clinic; Surgery, Mayo Clinic., Gessner RC; SonoVol, Inc., Czernuszewicz TJ; SonoVol, Inc., Kline TL; Radiology, Mayo Clinic., Romero MF; Physiology & Biomedical Engineering, Mayo Clinic; Nephrology & Hypertension, Mayo Clinic; romero.michael@mayo.edu. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of visualized experiments : JoVE [J Vis Exp] 2021 Aug 12 (174). Date of Electronic Publication: 2021 Aug 12. |
| DOI: |
10.3791/62682 |
| Abstrakt: |
Common modalities for in vivo imaging of rodents include positron emission tomography (PET), computed tomography (CT), magnetic resonance imaging (MRI), and ultrasound (US). Each method has limitations and advantages, including availability, ease of use, cost, size, and the use of ionizing radiation or magnetic fields. This protocol describes the use of 3D robotic US for in vivo imaging of rodent kidneys and heart, subsequent data analysis, and possible research applications. Practical applications of robotic US are the quantification of total kidney volume (TKV), as well as the measurement of cysts, tumors, and vasculature. Although the resolution is not as high as other modalities, robotic US allows for more practical high throughput data collection. Furthermore, using US M-mode imaging, cardiac function may be quantified. Since the kidneys receive 20%-25% of the cardiac output, assessing cardiac function is critical to the understanding of kidney physiology and pathophysiology. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
