Visualizing murine breast and melanoma tumor microenvironment using intravital multiphoton microscopy.
| Autor: | Jain R; Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2050, Australia., Tikoo S; Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2050, Australia., On K; Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2050, Australia., Martinez B; Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2050, Australia., Dervish S; Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2050, Australia., Cavanagh LL; Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2050, Australia., Weninger W; Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2050, Australia.; Department of Dermatology, Medical University of Vienna, Vienna 1090, Austria. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2021 Aug 19; Vol. 2 (3), pp. 100722. Date of Electronic Publication: 2021 Aug 19 (Print Publication: 2021). |
| DOI: | 10.1016/j.xpro.2021.100722 |
| Abstrakt: | Intravital multiphoton imaging of the tumor milieu allows for the dissection of intricate and dynamic biological processes in situ . Herein, we present a step-by-step protocol for setting up an experimental cancer imaging model that has been optimized for solid tumors such as breast cancer and melanoma implanted in the flanks of mice. This protocol can be utilized for dissecting tumor-immune cell dynamics in vivo or other tumor-specific biological questions. For complete details on the use of this protocol for intravital imaging of breast cancer, please refer to Tikoo et al. (2021a), and for intravital imaging of melanoma, please refer to Tikoo et al. (2021b). Competing Interests: The authors declare no competing interests. (© 2021 The Authors.) |
| Databáze: | MEDLINE |
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