Genetic fingerprinting and aflatoxin production of Aspergillus section Flavi associated with groundnut in eastern Ethiopia.
| Autor: | Mohammed A; School of Plant Science, College of Agriculture and Environmental Sciences, Haramaya University, P.O. Box 138, Dire Dawa, Ethiopia., Faustinelli PC; United States Department of Agriculture-Agricultural Research Service-National Peanut Research Laboratory, Dawson, GA, 39842-0509, USA., Chala A; College of Agriculture, Hawassa University, P.O. Box 5, Hawassa, Ethiopia., Dejene M; School of Plant Science, College of Agriculture and Environmental Sciences, Haramaya University, P.O. Box 138, Dire Dawa, Ethiopia., Fininsa C; School of Plant Science, College of Agriculture and Environmental Sciences, Haramaya University, P.O. Box 138, Dire Dawa, Ethiopia., Ayalew A; Partnership for Aflatoxin Control in Africa (PACA), African Union Commission, Addis Ababa, Ethiopia., Ojiewo CO; ICRISAT - Nairobi, UN-Avenue, Box 39063-00623, Nairobi, Kenya., Hoisington DA; College of Agriculture and Environmental Sciences, Peanut and Mycotoxin Innovation Lab, University of Georgia, Athens, GA, 30602-4356, USA., Sobolev VS; United States Department of Agriculture-Agricultural Research Service-National Peanut Research Laboratory, Dawson, GA, 39842-0509, USA., Martínez-Castillo J; Centro de Investigación Científica de Yucatán A.C., Unidad de Recursos Naturales, Calle 43 No. 130, Colonia Chuburná de Hidalgo CP 97200, Mérida, Mexico., Arias RS; United States Department of Agriculture-Agricultural Research Service-National Peanut Research Laboratory, Dawson, GA, 39842-0509, USA. renee.arias@usda.gov. |
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| Jazyk: | angličtina |
| Zdroj: | BMC microbiology [BMC Microbiol] 2021 Aug 28; Vol. 21 (1), pp. 239. Date of Electronic Publication: 2021 Aug 28. |
| DOI: | 10.1186/s12866-021-02290-3 |
| Abstrakt: | Background: Aspergillus species cause aflatoxin contamination in groundnut kernels, being a health threat in agricultural products and leading to commodity rejection by domestic and international markets. Presence of Aspergillus flavus and A. parasiticus colonizing groundnut in eastern Ethiopia, as well as presence of aflatoxins have been reported, though in this region, no genetic studies have been done of these species in relation to their aflatoxin production. Results: In this study, 145 Aspergillus isolates obtained from groundnut kernels in eastern Ethiopia were genetically fingerprinted using 23 Insertion/Deletion (InDel) markers within the aflatoxin-biosynthesis gene cluster (ABC), identifying 133 ABC genotypes. Eighty-four isolates were analyzed by Ultra-Performance Liquid Chromatography (UPLC) for in vitro aflatoxin production. Analysis of genetic distances based on the approximately 85 kb-ABC by Neighbor Joining (NJ), 3D-Principal Coordinate Analysis (3D-PCoA), and Structure software, clustered the isolates into three main groups as a gradient in their aflatoxin production. Group I, contained 98% A. flavus, including L- and non-producers of sclerotia (NPS), producers of B Conclusions: To the best of our knowledge, this is the first study that combined the use of InDel fingerprinting of the ABC and corresponding aflatoxin production capability to describe the genetic diversity of Aspergillus isolates from groundnut in eastern Ethiopia. Three InDel markers, AFLC04, AFLC08 and AFLC19, accounted for the main assignment of individuals to the three Groups; their loci corresponded to aflC (pksA), hypC, and aflW (moxY) genes, respectively. Despite InDels within the ABC being often associated to loss of aflatoxin production, the vast InDel polymorphism observed in the Aspergillus isolates did not completely impaired their aflatoxin production in vitro. (© 2021. The Author(s).) |
| Databáze: | MEDLINE |
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