Low dose of quercetin-loaded pectin/casein microparticles reduces the oxidative stress in arthritic rats.

Autor: Souza KS; Department of Biochemistry, State University of Maringa, PR, Brazil., Moreira LS; Department of Biochemistry, State University of Maringa, PR, Brazil., Silva BT; Department of Morphological Sciences, University of Maringá, PR, Brazil., Oliveira BPM; Post-Graduation Program of Food Technology - Federal University of Technology - Paraná, Campo Mourão, PR, Brazil., Carvalho AS; Post-Graduation Program of Food Technology - Federal University of Technology - Paraná, Campo Mourão, PR, Brazil., Silva PS; Department of Chemical Engineering, State University of Maringa, PR, Brazil., Verri WA Jr; Post-Graduation Program of Experimental Pathology, State University of Londrina, PR, Brazil., Sá-Nakanishi AB; Department of Biochemistry, State University of Maringa, PR, Brazil., Bracht L; Department of Biochemistry, State University of Maringa, PR, Brazil., Zanoni JN; Department of Morphological Sciences, University of Maringá, PR, Brazil., Gonçalves OH; Post-Graduation Program of Food Technology - Federal University of Technology - Paraná, Campo Mourão, PR, Brazil., Bracht A; Department of Biochemistry, State University of Maringa, PR, Brazil., Comar JF; Department of Biochemistry, State University of Maringa, PR, Brazil. Electronic address: jfcomar@uem.br.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2021 Nov 01; Vol. 284, pp. 119910. Date of Electronic Publication: 2021 Aug 25.
DOI: 10.1016/j.lfs.2021.119910
Abstrakt: Aims: Quercetin has been investigated as an agent to treat rheumatoid arthritis. At high doses it improves inflammation and the antioxidant status of arthritic rats, but it also exerts mitochondriotoxic and pro-oxidant activities. Beneficial effects of quercetin have not been found at low doses because of its chemical instability and low bioavailability. In the hope of overcoming these problems this study investigated the effects of long-term administration of quercetin-loaded pectin/casein microparticles on the oxidative status of liver and brain of rats with adjuvant-induced arthritis.
Main Methods: Particle morphology was viewed with transmission electron microscopy and the encapsulation efficiency was measured indirectly by X-ray diffraction. Quercetin microcapsules (10 mg/Kg) were orally administered to rats during 60 days. Inflammation indicators and oxidative stress markers were measured in addition to the respiratory activity and ROS production in isolated mitochondria.
Key Findings: Quercetin was efficiently encapsulated inside the polymeric matrix, forming a solid amorphous solution. The administration of quercetin microparticles to arthritic rats almost normalized protein carbonylation, lipid peroxidation, the levels of reactive oxygen species as well as the reduced glutathione content in both liver and brain. The paw edema in arthritic rats was not responsive, but the plasmatic activity of ALT and the mitochondrial respiration were not affected by quercetin, indicating absence of mitochondriotoxic or hepatotoxic actions.
Significance: Quercetin-loaded pectin/casein microcapsules orally administered at a low dose improve oxidative stress of arthritic rats without a strong anti-inflammatory activity. This supports the long-term use of quercetin as an antioxidant agent to treat rheumatoid arthritis.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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