Red blood cell derived extracellular vesicles during the process of autologous blood doping.

Autor: Voss SC; Anti-Doping Lab Qatar, Doha, Qatar., Yassin M; Hamad Medical Corporation, Doha, Qatar., Grivel JC; Sidra Medicine, Deep Phenotyping Core - Research Department, Doha, Qatar., Al Hmissi S; Hamad Medical Corporation, Doha, Qatar., Allahverdi N; Hamad Medical Corporation, Doha, Qatar., Nashwan A; Hamad Medical Corporation, Doha, Qatar., Merenkov Z; Hamad Medical Corporation, Doha, Qatar., Abdulla M; Hamad Medical Corporation, Doha, Qatar., Al Malki A; Hamad Medical Corporation, Doha, Qatar., Raynaud C; Sidra Medicine, Deep Phenotyping Core - Research Department, Doha, Qatar., Elsaftawy W; Anti-Doping Lab Qatar, Doha, Qatar., Al Kaabi A; Anti-Doping Lab Qatar, Doha, Qatar., Donati F; Anti-Doping Lab FMSI, Rome, Italy., Botre F; Anti-Doping Lab FMSI, Rome, Italy., Mohamed Ali V; Anti-Doping Lab Qatar, Doha, Qatar.; Centre for Metabolism and Inflammation, Division of Medicine, University College London, London, UK., Georgakopoulos C; Anti-Doping Lab Qatar, Doha, Qatar., Al Maadheed M; Anti-Doping Lab Qatar, Doha, Qatar.; Centre for Metabolism and Inflammation, Division of Medicine, University College London, London, UK.
Jazyk: angličtina
Zdroj: Drug testing and analysis [Drug Test Anal] 2022 Nov; Vol. 14 (11-12), pp. 1984-1994. Date of Electronic Publication: 2021 Sep 10.
DOI: 10.1002/dta.3157
Abstrakt: The purpose of this pilot study was to investigate the effects of the transfusion of one erythrocyte concentrate on the number of circulating red blood cell extracellular vesicles (RBC-EVs) and their clearance time. Six, healthy volunteers donated their blood and were transfused with their RBC concentrate after 35-36 days of storage. One K 2 EDTA and one serum sample were collected before donation, at four timepoints after donation and at another six timepoints after transfusion. RBC-EVs were analyzed on a Cytek Aurora flow cytometer. A highly significant increase (p < 0.001) of RBC-EVs from an average of 60.1 ± 19.8 (10 3 /μL) at baseline to 179.3 ± 84.7 (10 3 /μL) in the first 1-3 h after transfusion could be observed. Individual differences in the response to transfusion became apparent with one volunteer showing no increase and another an increased concentration at one timepoint after donation due to an influenza infection. We concluded that in an individualized passport approach, increased RBC-EVs might be considered as additional evidence when interpreting suspicious Athletes Biological Passport (ABPs) but for this additional research related to sample collection and transport processes as well as method development and harmonization would be necessary.
(© 2021 The Authors. Drug Testing and Analysis published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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