| Autor: |
Ospina Nieto CA; Centre for Crop Systems Analysis, Wageningen University, P.O. Box 430, 6700 AK Wageningen, The Netherlands.; Wageningen UR Plant Breeding, P.O. Box 386, 6700 AJ Wageningen, The Netherlands., Lammerts van Bueren ET; Wageningen UR Plant Breeding, P.O. Box 386, 6700 AJ Wageningen, The Netherlands., Allefs S; Agrico Research, Burchtweg 17, 8314 PP Bant, The Netherlands., Vos PG; Wageningen UR Plant Breeding, P.O. Box 386, 6700 AJ Wageningen, The Netherlands., van der Linden G; Wageningen UR Plant Breeding, P.O. Box 386, 6700 AJ Wageningen, The Netherlands., Maliepaard CA; Wageningen UR Plant Breeding, P.O. Box 386, 6700 AJ Wageningen, The Netherlands., Struik PC; Centre for Crop Systems Analysis, Wageningen University, P.O. Box 430, 6700 AK Wageningen, The Netherlands. |
| Abstrakt: |
Ample nitrogen (N) is required for potato production, but its use efficiency is low. N supply strongly interacts with maturity type of the cultivar grown. We assessed whether variation among 189 cultivars grown with 75 or 185 kg available N/ha in 2 years would allow detecting quantitative trait loci (QTLs) for relevant traits. Using phenotypic data, we estimated various traits and carried out a genome-wide association study (GWAS) with kinship correction. Twenty-four traits and 10,747 markers based on single-nucleotide polymorphisms from a 20K Infinium array for 169 cultivars were combined in the analysis. N level affected most traits and their interrelations and influenced the detection of marker-trait associations; some were N-dependent, others were detected at both N levels. Ninety percent of the latter accumulated on a hotspot on Chromosome 5. Chromosomes 2 and 4 also contained regions with multiple associations. After correcting for maturity, the number of QTLs detected was much lower, especially of those common to both N levels; however, interestingly, the region on Chromosome 2 accumulated several QTLs. There is scope for marker-assisted selection for maturity, with the main purpose of improving characteristics within a narrow range of maturity types, in order to break the strong links between maturity type and traits like N use efficiency. |
