| Autor: |
Jeong BY; Department of Crop Science, Konkuk University, Seoul 05029, Korea., Lee Y; Department of Crop Science, Konkuk University, Seoul 05029, Korea., Kwon Y; Department of Crop Science, Konkuk University, Seoul 05029, Korea., Kim JH; Department of Crop Science, Konkuk University, Seoul 05029, Korea., Ham TH; Department of Crop Science, Konkuk University, Seoul 05029, Korea., Kwon SW; Department of Plant Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang 50463, Korea., Lee J; Department of Crop Science, Konkuk University, Seoul 05029, Korea. |
| Abstrakt: |
A genome-wide association study (GWAS) was used to investigate the genetic basis of chilling tolerance in a collection of 117 rice accessions, including 26 Korean landraces and 29 weedy rices, at the reproductive stage. To assess chilling tolerance at the early young microspore stage, plants were treated at 12 °C for 5 days, and tolerance was evaluated using seed set fertility. GWAS, together with principal component analysis and kinship matrix analysis, revealed five quantitative trait loci (QTLs) associated with chilling tolerance on chromosomes 3, 6, and 7. The percentage of phenotypic variation explained by the QTLs was 11-19%. The genomic region underlying the QTL on chromosome 3 overlapped with a previously reported QTL associated with spikelet fertility. Subsequent bioinformatic and haplotype analyses suggested three candidate chilling-tolerance genes within the QTL linkage disequilibrium block: Os03g0305700, encoding a protein similar to peptide chain release factor 2; Os06g0495700, encoding a beta tubulin, autoregulation binding-site-domain-containing protein; and Os07g0137800, encoding a protein kinase, core-domain-containing protein. Further analysis of the detected QTLs and the candidate chilling-tolerance genes will facilitate strategies for developing chilling-tolerant rice cultivars in breeding programs. |
