Cartilaginous spheroid-assembly design considerations for endochondral ossification: towards robotic-driven biomanufacturing.
Autor: | Nilsson Hall G; Prometheus Division of Skeletal Tissue Engineering, KU Leuven, O&N1, Herestraat 49, PB 813, 3000 Leuven, Belgium.; Skeletal Biology and Engineering Research Center, Department of Development and Regeneration, KU Leuven, O&N1, Herestraat 49, PB 813, 3000 Leuven, Belgium., Rutten I; Department of Biosystems, Biosensors Group, KU Leuven, Willem de Croylaan 42, Box 2428, 3001 Leuven, Belgium., Lammertyn J; Department of Biosystems, Biosensors Group, KU Leuven, Willem de Croylaan 42, Box 2428, 3001 Leuven, Belgium., Eberhardt J; ALS Automated Lab Solutions GmbH, Jena, Germany., Geris L; Prometheus Division of Skeletal Tissue Engineering, KU Leuven, O&N1, Herestraat 49, PB 813, 3000 Leuven, Belgium.; GIGA in silico medicine, Université de Liège, Avenue de l'Hôpital 11-BAT 34, 4000 Liège 1, Belgium.; Biomechanics Section, KU Leuven, Celestijnenlaan 300C, PB 2419, 3001 Leuven, Belgium., Luyten FP; Prometheus Division of Skeletal Tissue Engineering, KU Leuven, O&N1, Herestraat 49, PB 813, 3000 Leuven, Belgium.; Skeletal Biology and Engineering Research Center, Department of Development and Regeneration, KU Leuven, O&N1, Herestraat 49, PB 813, 3000 Leuven, Belgium., Papantoniou I; Prometheus Division of Skeletal Tissue Engineering, KU Leuven, O&N1, Herestraat 49, PB 813, 3000 Leuven, Belgium.; Skeletal Biology and Engineering Research Center, Department of Development and Regeneration, KU Leuven, O&N1, Herestraat 49, PB 813, 3000 Leuven, Belgium.; Institute of Chemical Engineering Sciences, Foundation for Research and Technology-Hellas, Stadiou 26504, Platani, Patras, Greece. |
---|---|
Jazyk: | angličtina |
Zdroj: | Biofabrication [Biofabrication] 2021 Sep 21; Vol. 13 (4). Date of Electronic Publication: 2021 Sep 21. |
DOI: | 10.1088/1758-5090/ac2208 |
Abstrakt: | Spheroids have become essential building blocks for biofabrication of functional tissues. Spheroid formats allow high cell-densities to be efficiently engineered into tissue structures closely resembling the native tissues. In this work, we explore the assembly capacity of cartilaginous spheroids ( d ∼ 150 µ m) in the context of endochondral bone formation. The fusion capacity of spheroids at various degrees of differentiation was investigated and showed decreased kinetics as well as remodeling capacity with increased spheroid maturity. Subsequently, design considerations regarding the dimensions of engineered spheroid-based cartilaginous mesotissues were explored for the corresponding time points, defining critical dimensions for these type of tissues as they progressively mature. Next, mesotissue assemblies were implanted subcutaneously in order to investigate the influence of spheroid fusion parameters on endochondral ossification. Moreover, as a step towards industrialization, we demonstrated a novel automated image-guided robotics process, based on targeting and registering single-spheroids, covering the range of spheroid and mesotissue dimensions investigated in this work. This work highlights a robust and automated high-precision biomanufacturing roadmap for producing spheroid-based implants for bone regeneration. (Creative Commons Attribution license.) |
Databáze: | MEDLINE |
Externí odkaz: |