Molecular characterisation of parvorder Platyrrhini IgG sub-classes.

Autor: Yepes-Pérez Y; Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50 No. 26-20, Bogotá D.C. 111321, Colombia. Electronic address: yoelisyepes@gmail.com., Rodríguez-Obediente K; Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50 No. 26-20, Bogotá D.C. 111321, Colombia; MSc Programme in Microbiology, Universidad Nacional de Colombia, Carrera 45#26-85, Bogotá D.C. 111321, Colombia. Electronic address: krodriguezo@unal.edu.co., Camargo A; Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50 No. 26-20, Bogotá D.C. 111321, Colombia. Electronic address: annycam93@gmail.com., Diaz-Arévalo D; Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50 No. 26-20, Bogotá D.C. 111321, Colombia. Electronic address: dianadiazarevalo@gmail.com., Patarroyo ME; Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50 No. 26-20, Bogotá D.C. 111321, Colombia; Pathology Department, Faculty of Medicine, Universidad Nacional de Colombia, Carrera 45#26-85, Bogotá D.C. 111321, Colombia; Health Sciences Division, Main Campus, Universidad Santo Tomás, Carrera 9#51-11, Bogotá D.C. 110231, Colombia. Electronic address: mepatarr@gmail.com., Patarroyo MA; Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50 No. 26-20, Bogotá D.C. 111321, Colombia; Health Sciences Division, Main Campus, Universidad Santo Tomás, Carrera 9#51-11, Bogotá D.C. 110231, Colombia; Microbiology Department, Faculty of Medicine, Universidad Nacional de Colombia, Carrera 45#26-85, Bogotá D.C. 111321, Colombia. Electronic address: mapatarr.fidic@gmail.com.
Jazyk: angličtina
Zdroj: Molecular immunology [Mol Immunol] 2021 Nov; Vol. 139, pp. 23-31. Date of Electronic Publication: 2021 Aug 25.
DOI: 10.1016/j.molimm.2021.08.012
Abstrakt: Non-human primates (NHP) are essential in modern biomedical research; New World monkeys (NWM) are mainly used as an experimental model regarding human malaria as they provide useful information about the parasite's biology and an induced immune response. It is known that a vaccine candidate's efficacy is mediated by a protection-inducing antibody response (IgG). Not enough information is available concerning IgG subclasses' molecular characteristics regarding NHP from parvorder Platyrrhini. Understanding the nature of the humoral immune response and characterising the IgG subclasses' profile will provide valuable information about the immunomodulator mechanisms of vaccines evaluated using an NHP animal model. This article has characterised IgG subclasses in NWM (i.e. genera Aotus, Cebus, Ateles and Alouatta) based on the amplification, cloning and sequencing of the immunoglobulin heavy constant gamma (IGHG) gene's CH1 to CH3 regions. The resulting sequences enabled elucidating IGHG gene organisation; two IgG variants were found in the Aotus and Ateles monkey group and three IgG variants in the Cebus and Alouatta group. The sequences were highly conserved in Platyrrhini and had a similar structure to that reported for monkeys from parvorder Catarrhini. Such information will help in developing tools for a detailed characterisation of the humoral immune response in an NWM experimental animal model.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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