The potential of the incorporated collagen microspheres in alginate hydrogel as an engineered three-dimensional microenvironment to attenuate apoptosis in human pancreatic islets.

Autor: Kaviani M; Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran., Keshtkar S; Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran., Sarvestani FS; Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran., Azarpira N; Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: azarpiran@sums.ac.ir., Yaghobi R; Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran., Aghdaei MH; Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran., Geramizadeh B; Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran., Esfandiari E; Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran., Shamsaeefar A; Shiraz Organ Transplant Center, Shiraz University of Medical Sciences, Shiraz, Iran., Nikeghbalian S; Shiraz Organ Transplant Center, Shiraz University of Medical Sciences, Shiraz, Iran., Al-Abdullah IH; Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, Beckman Research Institute of City of Hope, Duarte, USA., Karimi MH; Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran., Motazedian N; Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Jazyk: angličtina
Zdroj: Acta histochemica [Acta Histochem] 2021 Oct; Vol. 123 (7), pp. 151775. Date of Electronic Publication: 2021 Aug 24.
DOI: 10.1016/j.acthis.2021.151775
Abstrakt: Background: Tissue engineering is considered as a promising tool for remodeling the native cells microenvironment. In the present study, the effect of alginate hydrogel and collagen microspheres integrated with extracellular matrix components were evaluated in the decrement of apoptosis in human pancreatic islets.
Materials/methods: For three-dimensional culture, the islets were encapsulated in collagen microspheres, containing laminin and collagen IV and embedded in alginate scaffold for one week. After that the islets were examined in terms of viability, apoptosis, genes and proteins expression including BAX, BCL2, active caspase-3, and insulin. Moreover, the islets function was evaluated through glucose-induced insulin and C-peptide secretion assay. In order to evaluate the structure of the scaffolds and the morphology of the pancreatic islets in three-dimensional microenvironments, we performed scanning electron microscopy.
Results: Our findings showed that the designed hydrogel scaffolds significantly improved the islets viability using the reduction of activated caspase-3 and TUNEL positive cells.
Conclusions: The reconstruction of the destructed matrix with alginate hydrogels and collagen microspheres might be an effective step to promote the culture of the islets.
(Copyright © 2021 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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