OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human.

Autor: Huang X; Department of Medicine, Columbia Center for Human Development, Columbia Stem Cell Initiative, Columbia University Irving Medical Center, New York, NY, USA., Park KM; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO, USA.; Center of Regenerative Medicine, Washington University School of Medicine, St. Louis, MO, USA., Gontarz P; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO, USA.; Center of Regenerative Medicine, Washington University School of Medicine, St. Louis, MO, USA., Zhang B; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO, USA.; Center of Regenerative Medicine, Washington University School of Medicine, St. Louis, MO, USA., Pan J; Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.; Broad Institute of Harvard and MIT, Cambridge, MA, USA., McKenzie Z; Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA, USA., Fischer LA; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO, USA.; Center of Regenerative Medicine, Washington University School of Medicine, St. Louis, MO, USA., Dong C; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO, USA.; Center of Regenerative Medicine, Washington University School of Medicine, St. Louis, MO, USA., Dietmann S; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO, USA.; Institute of Informatics (I2), Washington University School of Medicine, St. Louis, MO, USA., Xing X; Center of Regenerative Medicine, Washington University School of Medicine, St. Louis, MO, USA.; Department of Genetics, Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, MO, USA., Shliaha PV; Memorial Sloan Kettering Cancer Center, New York, NY, USA., Yang J; Department of Medicine, Columbia Center for Human Development, Columbia Stem Cell Initiative, Columbia University Irving Medical Center, New York, NY, USA., Li D; Department of Medicine, Columbia Center for Human Development, Columbia Stem Cell Initiative, Columbia University Irving Medical Center, New York, NY, USA.; Department of Cell, Developmental and Regenerative Biology, Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Ding J; Department of Cell, Developmental and Regenerative Biology, Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Department of Cell Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China., Lungjangwa T; Whitehead Institute for Biomedical Research, Cambridge, MA, USA., Mitalipova M; Whitehead Institute for Biomedical Research, Cambridge, MA, USA., Khan SA; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO, USA.; Center of Regenerative Medicine, Washington University School of Medicine, St. Louis, MO, USA., Imsoonthornruksa S; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.; Center for Biomolecular Structure Function and Application, School of Biotechnology, Suranaree University of Technology, Nakhon Ratchasima, Thailand., Jensen N; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO, USA.; Center of Regenerative Medicine, Washington University School of Medicine, St. Louis, MO, USA., Wang T; Center of Regenerative Medicine, Washington University School of Medicine, St. Louis, MO, USA.; Department of Genetics, Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, MO, USA., Kadoch C; Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.; Broad Institute of Harvard and MIT, Cambridge, MA, USA., Jaenisch R; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Wang J; Department of Medicine, Columbia Center for Human Development, Columbia Stem Cell Initiative, Columbia University Irving Medical Center, New York, NY, USA. jw3925@cumc.columbia.edu., Theunissen TW; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO, USA. t.theunissen@wustl.edu.; Center of Regenerative Medicine, Washington University School of Medicine, St. Louis, MO, USA. t.theunissen@wustl.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 Aug 26; Vol. 12 (1), pp. 5123. Date of Electronic Publication: 2021 Aug 26.
DOI: 10.1038/s41467-021-25107-3
Abstrakt: Understanding the molecular underpinnings of pluripotency is a prerequisite for optimal maintenance and application of embryonic stem cells (ESCs). While the protein-protein interactions of core pluripotency factors have been identified in mouse ESCs, their interactome in human ESCs (hESCs) has not to date been explored. Here we mapped the OCT4 interactomes in naïve and primed hESCs, revealing extensive connections to mammalian ATP-dependent nucleosome remodeling complexes. In naïve hESCs, OCT4 is associated with both BRG1 and BRM, the two paralog ATPases of the BAF complex. Genome-wide location analyses and genetic studies reveal that these two enzymes cooperate in a functionally redundant manner in the transcriptional regulation of blastocyst-specific genes. In contrast, in primed hESCs, OCT4 cooperates with BRG1 and SOX2 to promote chromatin accessibility at ectodermal genes. This work reveals how a common transcription factor utilizes differential BAF complexes to control distinct transcriptional programs in naïve and primed hESCs.
(© 2021. The Author(s).)
Databáze: MEDLINE
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