| Autor: |
Bogahawaththa S; Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya 20400, Sri Lanka.; The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan.; Laboratory of Biochemistry, Faculty of Agriculture, Saga University, Saga 840-8502, Japan., Kodithuwakku SP; Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya 20400, Sri Lanka., Wijesundera KK; Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya 20400, Sri Lanka., Siriweera EH; Department of Pathology, Faculty of Medicine, University of Peradeniya, Peradeniya 20400, Sri Lanka., Jayasinghe L; National Institute of Fundamental Studies, Hanthana Road, Kandy 20000, Sri Lanka., Dissanayaka WL; Applied Oral Sciences & Community Dental Care, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China., Rajapakse J; Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya 20400, Sri Lanka., Herath CB; Department of Medicine, Austin Health, Melbourne Medical School, The University of Melbourne, Heidelberg, VIC 3084, Australia.; South Western Sydney Clinical School and Ingham Institute for Applied Medical Research, Faculty of Medicine, University of New South Wales, Liverpool, NSW 2170, Australia., Tsujita T; The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan.; Laboratory of Biochemistry, Faculty of Agriculture, Saga University, Saga 840-8502, Japan., Wijayagunawardane MPB; Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya 20400, Sri Lanka. |
| Abstrakt: |
Chronic liver inflammation has become a major global health concern. In the absence of clinical surrogate markers to diagnose inflammatory liver disease, the intervention with effective drugs in modern medicine tends to be late. In Sri Lanka, traditional medical practitioners prescribe herbal preparations from Osbeckia octandra for the prevention and treatment of liver disorders. To test the efficacy of such treatments, we have administered thioacetamide (TAA) to male Wistar rats to induce chronic liver damage (disease control; DC) and examined how various leaf extracts: crude leaf suspension (CLS), boiled leaf extract (BLE), sonicated leaf extract (SLE), methanol leaf extract (MLE) and hexane leaf extract (HLE) of O. octandra ameliorate TAA-induced liver disease. The CLS, BLE and SLE treatments in cirrhotic rats significantly attenuated disease-related changes, such as liver weight and hepato-enzymes. The mRNA levels of Tnf-α were significantly decreased by 3.6, 10 and 3.9 times in CLS, BLE and SLE compared to DC. The same treatments resulted in significantly lower (19.5, 4.2 and 2.4 times) α-Sma levels compared to DC. In addition, Tgf-β1 and Vegf-R2 mRNA expressions were significantly lower with the treatments. Moreover, BLE expressed a strong anti-angiogenic effect. We conclude that CLS, BLE and SLE from O. octandra have potent hepatic anti-fibrotic effects in TAA-induced liver cirrhosis. |
