| Autor: |
Mulatu A; Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia., Alemu T; Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia., Megersa N; Department of Chemistry, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia., Vetukuri RR; Department of Plant Breeding, Swedish University of Agricultural Sciences, 230 53 Alnarp, Sweden. |
| Jazyk: |
angličtina |
| Zdroj: |
Microorganisms [Microorganisms] 2021 Aug 06; Vol. 9 (8). Date of Electronic Publication: 2021 Aug 06. |
| DOI: |
10.3390/microorganisms9081675 |
| Abstrakt: |
Agro-industrial wastes suitable for economical and high mass production of novel Trichoderma species under solid-state fermentation were identified by optimizing the culture conditions using a mathematical model and evaluating the viability of the formulated bio-product. Fourteen inexpensive, locally available, organic substrates and cereals were examined using a one-factor-at-a-time experiment. The fungus colonized nearly all substrates after 21 days of incubation, although the degree of colonization and conidiation varied among the substrates. A mixture of wheat bran and white rice (2:1 w/w ) was found to support maximum growth of T. asperellum AU131 (3.2 × 10 7 spores/g dry substrate) and T. longibrachiatum AU158 (3.5 × 10 7 spores/g dry substrate). Using a fractional factorial design, the most significant growth factors influencing biomass production were found to be temperature, moisture content, inoculum concentration, and incubation period ( p ≤ 0.05). Analysis of variance of a Box-Behnken design showed that the regression model was highly significant ( p ≤ 0.05) with F -values of 10.38 ( P = 0.0027, T. asperellum AU131) and 12.01 ( p < 0.0017, T. longibrachiatum AU158). Under optimal conditions, maximum conidia yield of log 10 (8.6) ( T. asperellum AU131) and log 10 (9.18) ( T. longibrachiatum ) were obtained. For wettable powder Trichoderma species formulations, it was possible to maintain conidial viability at room temperature (25 °C) for eight months at concentrations above 10 6 CFU/g. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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