Neurotransmitter Profiles Are Altered in the Gut and Brain of Mice Mono-Associated with Bifidobacterium dentium .

Autor: Luck B; Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA.; Department of Pathology, Texas Children's Hospital, Houston, TX 77030, USA., Horvath TD; Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA.; Department of Pathology, Texas Children's Hospital, Houston, TX 77030, USA., Engevik KA; Department of Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA., Ruan W; Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA.; Section of Gastroenterology, Hepatology, and Nutrition, Texas Children's Hospital, Houston, TX 77030, USA., Haidacher SJ; Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA.; Department of Pathology, Texas Children's Hospital, Houston, TX 77030, USA., Hoch KM; Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA.; Department of Pathology, Texas Children's Hospital, Houston, TX 77030, USA., Oezguen N; Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA.; Department of Pathology, Texas Children's Hospital, Houston, TX 77030, USA., Spinler JK; Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA.; Department of Pathology, Texas Children's Hospital, Houston, TX 77030, USA., Haag AM; Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA.; Department of Pathology, Texas Children's Hospital, Houston, TX 77030, USA., Versalovic J; Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA.; Department of Pathology, Texas Children's Hospital, Houston, TX 77030, USA., Engevik MA; Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA.; Department of Regenerative Medicine & Cell Biology, Medical University of South Carolina, Charleston, SC 29425, USA.
Jazyk: angličtina
Zdroj: Biomolecules [Biomolecules] 2021 Jul 23; Vol. 11 (8). Date of Electronic Publication: 2021 Jul 23.
DOI: 10.3390/biom11081091
Abstrakt: Background: Accumulating evidence indicates that the gut microbiota can synthesize neurotransmitters as well as impact host-derived neurotransmitter levels. In the past, it has been challenging to decipher which microbes influence neurotransmitters due to the complexity of the gut microbiota.
Methods: To address whether a single microbe, Bifidobacterium dentium, could regulate important neurotransmitters, we examined Bifidobacteria genomes and explored neurotransmitter pathways in secreted cell-free supernatant using LC-MS/MS. To determine if B. dentium could impact neurotransmitters in vivo, we mono-associated germ-free mice with B. dentium ATCC 27678 and examined fecal and brain neurotransmitter concentrations.
Results: We found that B. dentium possessed the enzymatic machinery to generate γ-aminobutyric acid (GABA) from glutamate, glutamine, and succinate. Consistent with the genome analysis, we found that B. dentium secreted GABA in a fully defined microbial media and elevated fecal GABA in B. dentium mono-associated mice compared to germ-free controls. We also examined the tyrosine/dopamine pathway and found that B. dentium could synthesize tyrosine, but could not generate L-dopa, dopamine, norepinephrine, or epinephrine. In vivo, we found that B. dentium mono-associated mice had elevated levels of tyrosine in the feces and brain.
Conclusions: These data indicate that B. dentium can contribute to in vivo neurotransmitter regulation.
Databáze: MEDLINE
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